Salami Adeola T, Adebimpe Mayokun A, Olagoke Olawande C, Iyiola Toluwalope O, Olaleye Samuel B
Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria, Santa Maria, Brasil.
J Food Biochem. 2020 Dec;44(12):e13501. doi: 10.1111/jfbc.13501. Epub 2020 Oct 6.
The interaction between ingested xenobiotics and the gastrointestinal epithelium influences the possibility of gut epithelial cytotoxicity and systemic toxicity. Potassium bromate (KBrO ) has been shown to perturb the central nervous system and it may be carcinogenic, albeit it is used as a food additive. This highlights the need to understand KBrO 's effect on the stomach epithelium. Here, we report the cytotoxic potential of KBrO in an ulcerated stomach, as well as possible cytoprotection by the polyphenol - protocatechuic acid. Potassium bromate (12.5 mg/kg) and protocatechuic acid (120 mg/kg) were administered orally while omeprazole (20 mg/kg) was used as standard. Potassium bromate exacerbated gastric ulcers, increased malonaldehyde levels, catalase, and sodium pump activities, but reduced nitric oxide levels. Potassium bromate further increased mast cell count in the muscularis mucosa, while inducing chronic inflammation and moderate angiogenesis in the gastric mucosa. Our results delineate KBrO -induced gastric epithelial cytotoxicity that is ameliorated by protocatechuic acid. PRACTICAL APPLICATIONS: Potassium bromate is a known food additive in the baking, brewing, and cheese-making process. Conversely, protocatechuic acid (3,4-dihydroxybenzoic acid) is the polyphenolic content of plants like Hibiscus sabdariffa L that are commonly consumed as herbal drink, food, spices, and used in folk medicine. This study reports the cytoprotective effect of protocatechuic acid against gastric mucosa ulceration that has been aggravated by potassium bromate.
摄入的外源性物质与胃肠道上皮之间的相互作用会影响肠道上皮细胞毒性和全身毒性的可能性。溴酸钾（KBrO₃）已被证明会扰乱中枢神经系统，尽管它被用作食品添加剂，但可能具有致癌性。这凸显了了解溴酸钾对胃上皮细胞影响的必要性。在此，我们报告了溴酸钾在溃疡胃中的细胞毒性潜力，以及多酚原儿茶酸可能的细胞保护作用。口服给予溴酸钾（12.5毫克/千克）和原儿茶酸（120毫克/千克），同时使用奥美拉唑（20毫克/千克）作为对照标准。溴酸钾加剧了胃溃疡，增加了丙二醛水平、过氧化氢酶和钠泵活性，但降低了一氧化氮水平。溴酸钾进一步增加了肌层黏膜中的肥大细胞数量，同时在胃黏膜中诱导慢性炎症和中度血管生成。我们的结果描述了溴酸钾诱导的胃上皮细胞毒性，而原儿茶酸可改善这种毒性。实际应用：溴酸钾是烘焙、酿造和奶酪制作过程中一种已知的食品添加剂。相反，原儿茶酸（3,4 - 二羟基苯甲酸）是玫瑰茄等植物中的多酚成分，这些植物通常作为草药饮品、食物、香料食用，并用于民间医学。本研究报告了原儿茶酸对溴酸钾加重的胃黏膜溃疡的细胞保护作用。
