Institut de Médecine Légale, Strasbourg, France.
Centre hospitalier de Molsheim, Molsheim, France.
J Forensic Sci. 2021 Jan;66(1):272-277. doi: 10.1111/1556-4029.14585. Epub 2020 Oct 7.
Testing for drugs in hair raises several difficulties. Among them is the interpretation of the final concentration(s). In a post-mortem case, analyses revealed the presence of furosemide (12 ng/mL) in femoral blood, although it was not part of the victim's treatment. The prosecutor requested our laboratory to undertake an additional analysis in hair to obtain information about the use of furosemide. A specific method was therefore developed and validated to identify and quantify furosemide in hair by UHPLC-MS/MS. After decontamination of 30 mg of hair, incubation in acidic condition, extraction with ethyl acetate, the samples were analyzed by UHPLC-MS/MS. Furosemide was found in the victim's hair at 225 pg/mg. However, it was not possible to interpret this concentration due to the absence of data in the literature. Therefore, the authors performed a controlled study in two parts. In order to establish the basis of interpretation, several volunteers were tested (four after a single 20 mg administration and twenty-four under daily treatment). The first part indicated that a single dose is not detectable in hair using our method. The second part demonstrated concentrations ranging from 5 to 1110 pg/mg with no correlation between dosage and hair concentrations. The decedent's hair result was interpreted as repeated exposures. In the case of furosemide analysis, hair can provide information about its presence but cannot give information about dosage or frequency of use.
在头发中检测药物会带来一些困难。其中之一是对最终浓度的解释。在一个死后案例中，分析显示股动脉血液中存在呋塞米（12ng/ml），尽管它不是受害者治疗的一部分。检察官要求我们实验室对头发进行额外分析，以获取有关呋塞米使用情况的信息。因此，开发并验证了一种特定的方法，通过 UHPLC-MS/MS 来鉴定和定量头发中的呋塞米。在对 30mg 头发进行去污、酸性孵育、乙酸乙酯提取后，用 UHPLC-MS/MS 分析样品。在受害者的头发中发现了 225pg/mg 的呋塞米。然而，由于文献中缺乏数据，无法解释这个浓度。因此，作者进行了两部分的对照研究。为了建立解释的基础，对几名志愿者进行了测试（四名志愿者在单次 20mg 给药后进行检测，二十四名志愿者在每日治疗下进行检测）。第一部分表明，我们的方法不能检测单次剂量在头发中的存在。第二部分表明，浓度范围从 5 到 1110pg/mg，剂量与头发浓度之间没有相关性。死者头发的结果被解释为重复暴露。在呋塞米分析中，头发可以提供关于其存在的信息，但不能提供关于剂量或使用频率的信息。
