Department of Pharmacology, College of Korean Medicine, Sangji University, 83, Sangjidae-gil, Wonju-si, Gangwon-do, 26339, Republic of Korea.
Department of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Sangji University, 83, Sangjidae-gil, Wonju-si, Gangwon-do, 26339, Republic of Korea.
BMC Complement Med Ther. 2020 Oct 7;20(1):302. doi: 10.1186/s12906-020-03073-5.
Macrophages play a crucial role in inflammation. Astilbe chinensis is one of perennial herbs belonging to the genus Astilbe. Plants in the genus have been used for pain, headaches, arthralgia, and chronic bronchitis. However, the effect of A.chinensis on inflammation remains unclear. To study the anti-inflammatory action of A.chinensis ethanol extract (ACE), we investigated the effect of ACE on the production of pro-inflammatory mediators and cytokines in macrophages.
We evaluated the effectiveness of ACE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and thioglycollate (TG)-elicited peritoneal macrophages from male C57BL/6 mice. We measured the levels of pro-inflammatory mediators and cytokines, and examined the anti-inflammatory actions of ACE on nuclear factor κB (NF-κB) pathway in the macrophages. Western blot analysis and immunofluorescence microscopy were used to determine protein level and translocation, respectively.
ACE suppressed the output of nitric oxide (NO), prostaglandin E2 (PGE), and pro-inflammatory cytokines in stimulated macrophages via inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins. ACE suppressed mRNA expression of pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). We examined the efficacies of ACE on NF-κB activation by measuring the expressions including IκB kinase (IKK), inhibitor of κB (IκB), and nuclear p65 proteins. In addition, the inhibition of NF-κB p65's translocation was determined with immunofluorescence assay.
Our findings manifested that ACE inhibited LPS or TG-induced inflammation by blocking the NF-κB signaling pathway in macrophages. It indicated that ACE is a potential therapeutic mean for inflammation and related diseases.
巨噬细胞在炎症中起着至关重要的作用。 紫菀是紫菀属的多年生草本植物之一。 该属植物已用于治疗疼痛，头痛，关节痛和慢性支气管炎。 然而，紫菀属植物对炎症的影响尚不清楚。 为了研究紫菀属植物乙醇提取物（ACE）的抗炎作用，我们研究了 ACE 对巨噬细胞中促炎介质和细胞因子产生的影响。
我们评估了 ACE 在脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞和巯基乙酸（TG）诱导的雄性 C57BL / 6 小鼠腹腔巨噬细胞中的作用。我们测量了促炎介质和细胞因子的水平，并研究了 ACE 对巨噬细胞中核因子κB（NF-κB）途径的抗炎作用。 蛋白质水平和转位分别通过Western blot 分析和免疫荧光显微镜检查来确定。
ACE 通过抑制诱导型一氧化氮合酶（iNOS）和环加氧酶-2（COX-2）蛋白的表达，抑制刺激的巨噬细胞中一氧化氮（NO），前列腺素 E2（PGE）和促炎细胞因子的产生。 ACE 抑制白细胞介素（IL）-6和肿瘤坏死因子-α（TNF-α）等促炎细胞因子的mRNA 表达。 我们通过测量包括 IκB 激酶（IKK），κB 抑制剂（IκB）和核 p65 蛋白在内的表达来检查 ACE 对 NF-κB 激活的功效。 此外，通过免疫荧光测定法确定了 NF-κB p65 易位的抑制作用。
我们的研究结果表明，ACE 通过阻断巨噬细胞中的 NF-κB 信号通路抑制 LPS 或 TG 诱导的炎症。 这表明 ACE 是炎症和相关疾病的潜在治疗方法。
