Tolliver P R, Roe M H, Todd J K
Pediatr Infect Dis J. 1987 Jun;6(6):515-9.
Eight hundred thirty-seven consecutive throat cultures from pediatric patients were cultured comparatively on conventional sheep blood agar and selective agar containing 1.25 micrograms/ml trimethoprim and 23.75 micrograms/ml sulfamethoxazole. In two sequential studies the sheep blood agar-sulfamethoxazole-trimethoprim plates were significantly (P less than 0.01) more sensitive for Group A Streptococcus (Strep-A) detection than conventional sheep blood agar plates or Todd-Hewitt broth using the fluorescent antibody-antigen detection technique. The selective plates inhibited normal upper respiratory flora but not Strep-A. An incubation time of 18 hours was significantly better than one of 4 hours for the detection of Strep-A antigen in cultures incubated in Todd-Hewitt broth alone or Todd-Hewitt-sulfamethoxazole-trimethoprim broth, resulting in a 24% increase in positives. These studies demonstrate that Strep-A detection in throat swab specimens, by either culture or antigen detection, will be influenced by the use of selective antibiotics and incubation time.
对837份来自儿科患者的连续咽喉培养样本，分别在传统绵羊血琼脂培养基和含有1.25微克/毫升甲氧苄啶及23.75微克/毫升磺胺甲恶唑的选择性培养基上进行比较培养。在两项连续研究中，使用荧光抗体-抗原检测技术时，绵羊血琼脂-磺胺甲恶唑-甲氧苄啶平板对A组链球菌（链球菌A）检测的敏感性显著高于（P<0.01）传统绵羊血琼脂平板或托德-休伊特肉汤培养基。选择性平板抑制了正常上呼吸道菌群，但不抑制链球菌A。对于单独在托德-休伊特肉汤或托德-休伊特-磺胺甲恶唑-甲氧苄啶肉汤中培养的样本，检测链球菌A抗原时，18小时的孵育时间显著优于4小时，阳性率提高了24%。这些研究表明，无论是通过培养还是抗原检测来检测咽喉拭子样本中的链球菌A，都会受到选择性抗生素的使用和孵育时间的影响。
