Hassan Ibrahim, Wan Ibrahim Wan Norhamidah, Yusuf Ferdaus Mohamat, Ahmad Siti Aqlima, Ahmad Syahida
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Seri Kembangan 43400, Selangor, Malaysia.
Department of Biology, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Seri Kembangan 43400, Selangor, Malaysia.
J Toxicol. 2020 Sep 23;2020:8815313. doi: 10.1155/2020/8815313. eCollection 2020.
Pathophysiological changes leading to the death of nerve cells present in the brain and spinal cord are referred to as neurodegenerative diseases. Presently, treatment of these diseases is not effective and encounters many challenges due to the cost of drug and side effects. Thus, the search for the alternative agents to replace synthetic drugs is in high demand. Therefore, the aim of this study is to evaluate the anticholinesterase properties of seed.
The seed was extracted with 80% methanol. Toxicity studies and evaluation of anticholinesterase activities were carried out in adult Javanese medaka (). Phytochemical study to identify the bioactive lead constituents of the crude extract was also carried out using high performance liquid chromatography (HPLC).
The result shows activities with high significant differences at < 0.001 between the treated and nontreated groups. A bioactive compound (vitaxin) was identified with the aid of HPLC method.
The presence of bioactive compound vitaxin is among the major secondary metabolites that contribute to increasing activities of this plant extract. High anticholinesterase activities and low toxicity effect of this plant show its benefit to be used as natural medicine or supplements.
导致脑和脊髓中神经细胞死亡的病理生理变化被称为神经退行性疾病。目前，这些疾病的治疗效果不佳，且由于药物成本和副作用而面临诸多挑战。因此，对替代合成药物的替代剂的需求很高。因此，本研究的目的是评估种子的抗胆碱酯酶特性。
用80%甲醇提取种子。在成年爪哇青鳉中进行毒性研究和抗胆碱酯酶活性评估。还使用高效液相色谱法（HPLC）进行了植物化学研究，以鉴定粗提取物的生物活性主要成分。
结果显示，处理组和未处理组之间在P<0.001时有高度显著差异的活性。借助HPLC方法鉴定出一种生物活性化合物（维他新）。
生物活性化合物维他新的存在是导致该植物提取物活性增加的主要次生代谢产物之一。该植物的高抗胆碱酯酶活性和低毒性作用表明其有利于用作天然药物或补充剂。
