Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Serdang, Selangor, Malaysia.
Department of Biology, Faculty of Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Pak J Pharm Sci. 2021 Jan;34(1):47-56.
Diseases caused by oxidative stress can be prevented by antioxidant. Current treatments for those neurodegenerative diseases are not effective and cause many side effects. Thus, the search for alternative medicines is in high demand. Therefore, the main purposed of this study is to evaluate the neuroprotective effects of Curcuma longa (rhizome) 80% methanol extract. Antioxidant using dichlorofuoresence diacetate (DCF-DA) assay on SH-SY5Y cells revealed high activities of Curcuma longa (rhizome) extract with IC50 of 105.9±0.8 µg/mL. Sub-acute and chronic toxicity tests of the plant extract on adult Javanese medaka (Oryzias javanicus) showed high toxicity effect with LC of 24.15±0.8 mg/L and 13.69±0.7 mg/L respectively. Neuroprotective tests using cholinesterase assay disclose significant differences at P<0.05 between the group that are exposed to arsenic and treated with the crude extract and the group that are exposed to only arsenic. Identification of vitexin and isovitexin justified the high antioxidant potential of this plant leaf and it highest benefit to be used as medicinal supplement.
由氧化应激引起的疾病可以通过抗氧化剂来预防。目前针对这些神经退行性疾病的治疗方法并不有效，并且会引起许多副作用。因此，对替代药物的需求很高。因此，本研究的主要目的是评估姜黄（根茎）80%甲醇提取物的神经保护作用。使用二氯荧光素二乙酸酯（DCF-DA）测定法在 SH-SY5Y 细胞上进行抗氧化测定，结果表明姜黄（根茎）提取物具有高活性，IC50 为 105.9±0.8 µg/mL。对成年爪哇泥鳅（Oryzias javanicus）进行植物提取物的亚急性和慢性毒性测试表明，该植物提取物具有高毒性作用，LC 分别为 24.15±0.8 mg/L 和 13.69±0.7 mg/L。使用胆碱酯酶测定法进行的神经保护测试表明，暴露于砷并经粗提取物处理的组与仅暴露于砷的组之间存在显著差异（P<0.05）。牡荆素和异牡荆素的鉴定证明了这种植物叶子具有很高的抗氧化潜力，它是作为药用补充剂的最佳选择。
