Department of Orthopedics and Traumatology, Faculty of Medicine, Hacettepe University, 06230, Ankara, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Istanbul Aydin University, 34295, Istanbul, Turkey.
Tissue Eng Regen Med. 2020 Dec;17(6):901-908. doi: 10.1007/s13770-020-00298-w. Epub 2020 Oct 8.
Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model.
An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 × 10 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n = 14). The DHP scaffold, 1 × 10 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology.
The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p < 0.001, p < 0001, respectively). There was no statistically difference in mean Pineda scores of P, PS, PP, and PSP groups (p > 0.05).
In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
由于关节软骨自我再生能力有限，关节软骨修复一直是骨科实践中的一个挑战。尚未定义软骨缺损的最佳治疗方法。我们研究了去细胞人胎盘（DHP）支架、间充质干细胞（MSC）和富含血小板的血浆（PRP）在大鼠模型中对透明软骨再生的影响。
所有组的股骨滑车区域均双侧形成骨软骨缺损。对照组（n=14）未进行其他操作。仅在 P 组中应用 DHP 支架（n=14）。将 DHP 支架和 1×10 MSC 应用于 PS 组（n=14）。将 DHP 支架和 PRP 应用于 PP 组（n=14）。将 DHP 支架、1×10 MSC 和 PRP 应用于 PSP 组（n=14）。12 周时的主要终点包括 Pineda 组织学评分和定性组织学。
P、PS、PP 和 PSP 组的平均 Pineda 评分明显优于对照组（p=0.031，p=0.002，p<0.001，p<0.0001）。P、PS、PP 和 PSP 组的平均 Pineda 评分无统计学差异（p>0.05）。
总之，DHP 支架似乎是一种有前途的透明软骨再生支架。DHP 支架与 MSC 和 PRP 联合增强并不能提高其在关节软骨再生中的疗效。
