Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
Appl Immunohistochem Mol Morphol. 2020 Oct;28(9):678-686. doi: 10.1097/PAI.0000000000000818.
The present study aimed to investigate the expression levels and clinical significance of the calcineurin B homologous protein 2 (CHP2) in non-small cell lung cancer (NSCLC), and to study its effects on biological characteristics of NSCLC cells. Tumor and adjacent samples were collected from 196 NSCLC patients. Western blot analysis was used to detect the expression levels of the CHP2 in 8 pairs of NSCLC fresh tissues and 4 NSCLC cell lines. Immunohistochemical analysis was used to detect the expression of the CHP2 in 188 additional pairs of NSCLC wax block tissues. The data indicated that the expression levels of the CHP2 in the paraffin and fresh tissues of NSCLC were significantly higher than those of the adjacent tissues. According to the histo-score, univariate and multivariate analysis indicated that a high expression level of CHP2 was an important factor affecting the 5-year survival rate of NSCLC patients. After knocking down the expression of CHP2 in NSCLC cell lines, the proliferative, migratory, and invasive activities of NSCLC-CHP2 cells were decreased which were assessed by Western blotting, Cell Counting Kit-8, and transwell and wound-healing assays. In conclusion, the data demonstrated that CHP2 was highly expressed in NSCLC and that it could promote the development of NSCLC, suggesting its potential application for the therapy of NSCLC.
本研究旨在探讨钙调神经磷酸酶同源蛋白 2（CHP2）在非小细胞肺癌（NSCLC）中的表达水平及其临床意义，并研究其对 NSCLC 细胞生物学特性的影响。收集了 196 例 NSCLC 患者的肿瘤和相邻样本。Western blot 分析用于检测 8 对 NSCLC 新鲜组织和 4 种 NSCLC 细胞系中 CHP2 的表达水平。免疫组织化学分析用于检测 188 对额外的 NSCLC 蜡块组织中 CHP2 的表达。数据表明，CHP2 在 NSCLC 石蜡和新鲜组织中的表达水平明显高于相邻组织。根据组织评分，单因素和多因素分析表明，CHP2 高表达是影响 NSCLC 患者 5 年生存率的重要因素。在 NSCLC 细胞系中敲低 CHP2 的表达后，通过 Western blot、细胞计数试剂盒-8 检测和 Transwell 和划痕愈合实验评估 NSCLC-CHP2 细胞的增殖、迁移和侵袭活性均降低。总之，数据表明 CHP2 在 NSCLC 中高度表达，并且可以促进 NSCLC 的发展，提示其在 NSCLC 治疗中的潜在应用。
