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[噬菌体T4B琥珀突变体的重组。II. 琥珀突变体在基因30、34、35、36和38图谱上的定位]

[Recombination of amber mutants of bacteriophage T4B. II. Localization of amber mutants on maps of genes 30, 34, 35, 36 and 38].

作者信息

Piruzian E S, Zlotina S N

出版信息

Genetika. 1977;13(3):502-8.

PMID:330318
Abstract

Localization of 275 amber mutants of five genes of phage T4B (30, 34, 35, 36 and 38) on genetic maps allowed us to determine the recombination length of these genes. Gene 34 substantially differs from the rest studied genes by numbers of amber mutants isolated in each gene and by recombination frequency. In particular, according to the results of crossing the flank markers, the recombination length of gene 34 is 10 times greater than in gene 38; using the summary value of recombination frequencies between elementary intervals a 20-fold excess of the length of gene 34 compared with the length of gene 38 was receieved. Molecular weight of the product of gene 34 is only 6 times as great as in gene 38. An elevated recombination frequency was also detected in gene 35. The data obtained indicate a local recombination anomaly at the region of genes 34--35 of bacteriophage T4 genome.

摘要

噬菌体T4B五个基因(30、34、35、36和38)的275个琥珀突变体在遗传图谱上的定位,使我们能够确定这些基因的重组长度。基因34在每个基因中分离出的琥珀突变体数量和重组频率方面,与其他研究的基因有很大不同。特别是,根据侧翼标记杂交的结果,基因34的重组长度比基因38大10倍;利用基本区间之间重组频率的汇总值,发现基因34的长度比基因38长20倍。基因34产物的分子量仅为基因38的6倍。在基因35中也检测到重组频率升高。所获得的数据表明噬菌体T4基因组34 - 35基因区域存在局部重组异常。

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