Gordeev V K, Slutskiĭ A M
Genetika. 1978 Sep;14(9):1513-20.
Recombination frequencies between rII markers of bacteriophage T4 under conditions of partial inhibition of several early functions of the phage by amber mutations have been studied. Mutations in genes 33, 42 and 45 did not affect significantly recombination frequencies. Mutations in genes 32, 44 and 46 inhibited it. It has been found that effects of double mutations in pairs of genes: 43 and 30; 43 and 52; 43 and 33; 43 and 46; 43 and 32 were consistent with theoretically expected additive effects of corresponding single mutations. The presence of double mutations in pairs of genes: 43 and 41; 43 and 42, 43 and 44, 43 and 45 caused evident deviations from additivity. These deviations could reflect specific interactions of respective pairs of genes products under phage T4 recombination. Possible mechanisms of some of the effects observed are discussed.
在噬菌体T4的几种早期功能因琥珀突变而受到部分抑制的条件下，研究了噬菌体T4的rII标记之间的重组频率。基因33、42和45中的突变对重组频率没有显著影响。基因32、44和46中的突变则抑制了重组频率。已发现基因对43和30、43和52、43和33、43和46、43和32中的双突变效应与相应单突变的理论预期加性效应一致。基因对43和41、43和42、43和44、43和45中的双突变存在明显的非加性偏差。这些偏差可能反映了噬菌体T4重组过程中各基因对产物之间的特异性相互作用。文中讨论了部分观察到的效应的可能机制。
