Piruzian E S, Zlotina S N
Genetika. 1977;13(3):509-17.
Study was made of the influence of X-mutation on interallelic and intergenic recombination at the region of genes 34-38 of phage T4B. In the case of intergenic crosses in all studied recombinations of genes mutation X caused a 2-fold decrease in recombination frequency. X-mutation showed the same effect on integenic recombination in genes 36 and 38. In the case of crossing flank markers of gene 35 the frequency of recombination decreased 3.5-fold, that of gene 34--6 fold. Crosses of double am-X mutants mapped at different sites of gene 34 allowed to detect the gradient of the effect X-mutation on recombination in gene 34 in the direction from gene 33 to gene 35. The data obtained indicate a local anomaly of DNA structure of the region of genes 34 and 35 and allowed to draw a conclusion that the degree of suppressing recombination process under the influence of X-mutation depends nor on the original recombination frequency between markers to be crossed, that is on the internal structure of the given region of the genome of bacteriophage T4. Possible reasons for specific effect of X-mutations on the recombination in genes 34 and 35 of phage T4B are discussed.
研究了X突变对噬菌体T4B基因34 - 38区域内等位基因间和基因间重组的影响。在所有研究的基因间杂交中，X基因突变导致重组频率降低了2倍。X突变对基因36和38中的基因内重组也有相同的影响。在对基因三十五的侧翼标记进行杂交时，重组频率降低了3.5倍，基因三十四降低了6倍。对位于基因34不同位点的双am - X突变体进行杂交，能够检测到X突变对基因34中从基因33到基因35方向重组的影响梯度。所获得的数据表明基因34和35区域的DNA结构存在局部异常，并得出结论：在X突变影响下重组过程的抑制程度既不取决于待杂交标记之间的原始重组频率，即不取决于噬菌体T4基因组给定区域的内部结构。文中还讨论了X突变对噬菌体T4B基因34和35重组产生特定影响的可能原因。
