基于金纳米粒子比色适体传感器测定 17β-雌二醇的截断亲和改进适体。
Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor.
机构信息
Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Zhongguancun South Street No. 12, Haidian, Beijing 100081, China.
Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Middle Road of Shuguanghuayuan, Haidian, Beijing 100097, China.
出版信息
Food Chem. 2021 Mar 15;340:128181. doi: 10.1016/j.foodchem.2020.128181. Epub 2020 Sep 25.
17β-estradiol (E2) residues could enrich in organisms via food chain and lead to harmful biological effects for human body. To ascertain the binding domain of original E2 aptamer (E00) with long-sequence (76-mer), we developed novel truncated aptamers from E00, through rationally designed truncation by intercepting a single ring or a combination of rings (containing hairpin loop, interior loop or multiloop) at different sites and retaining appropriate double helix regions. Through comparison, 15-mer E09 presented improved affinity and higher specificity, indicating the hairpin loop near to 3' end of E00 served on the binding domain to E2. E09 was used for gold nanoparticles (AuNPs)-based colorimetric determination of E2, achieved the detection limit of 0.02 μg/mL. The truncated aptamer (only 15-mer) first proposed in this study has great application potential in E2 determination, and this work provides proof-of-concept study for truncation of other long-sequence aptamers.
17β-雌二醇（E2）残留可以通过食物链在生物体内富集，对人体产生有害的生物学效应。为了确定原始 E2 适体（E00）与长序列（76 -mer）的结合域，我们通过在不同位点拦截单个环或多个环（包含发夹环、内环或多环）并保留适当的双螺旋区域，从 E00 中设计合理地截短来开发新的截断适体。通过比较，15 -mer E09 表现出改善的亲和力和更高的特异性，表明 E00 近 3' 端的发夹环作为与 E2 的结合域。E09 用于基于金纳米粒子（AuNPs）的 E2 比色测定，检测限为 0.02μg/mL。本研究首次提出的截断适体（仅 15-mer）在 E2 测定中具有很大的应用潜力，这项工作为其他长序列适体的截断提供了概念验证研究。
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