School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Biotechnol Appl Biochem. 2023 Jun;70(3):1384-1396. doi: 10.1002/bab.2447. Epub 2023 Feb 13.
Trace of 17β-estradiol (E2) contamination in food has been a concern for its negative impacts on human health, leading to the need for an E2-monitoring system. This work reported a new simple, sensitive, and colorimetric E2 detection based on the designed repetitive-loop aptamer and gold nanoparticles (AuNPs). The designed aptamers (L2-L5) exhibited a higher binding capability to E2 than the original truncated aptamer (L1). Although L3-L5 aptamers exhibited the highest binding capability, only L3-aptasensor demonstrated the sensitive detection of E2 in a range of 0.05-0.8 nM, with the limit of detection at 13.1 pM. The developed L3-aptasensor was 7.7-folds more sensitive for E2 detection than the L1-aptasensor. It selectively detected E2, but not the other tested chemicals with similar structures: progesterone, genistein, diethylstilbestrol, bisphenol A, and chloramphenicol. The L3-aptasensor efficiently detected E2 spiked in milk samples within the precision acceptance criterion of recovery rates (100.1%-113.0%) and the relative standard deviations (5.24%-11.06%). These results demonstrated the development of a new aptasensor based on the designed repetitive-loop aptamer that could enhance E2-detection sensitivity and be potentially used for detecting E2 in milk samples with high accuracy and reliability.
痕量 17β-雌二醇（E2）污染一直是人们关注的焦点，因为它对人类健康有负面影响，这就需要建立一个 E2 监测系统。本工作报道了一种新的简单、灵敏、比色 E2 检测方法，基于设计的重复环适体和金纳米粒子（AuNPs）。所设计的适体（L2-L5）比原始截断适体（L1）具有更高的与 E2 的结合能力。虽然 L3-L5 适体表现出最高的结合能力，但只有 L3-适体传感器在 0.05-0.8 nM 的范围内表现出对 E2 的灵敏检测，检测限为 13.1 pM。与 L1-适体传感器相比，开发的 L3-适体传感器对 E2 的检测灵敏度提高了 7.7 倍。它选择性地检测 E2，但不检测其他具有相似结构的测试化学品：孕酮、染料木黄酮、己烯雌酚、双酚 A 和氯霉素。L3-适体传感器能够高效地检测牛奶样品中添加的 E2，回收率（100.1%-113.0%）和相对标准偏差（5.24%-11.06%）均在精度验收标准范围内。这些结果表明，基于设计的重复环适体开发了一种新的适体传感器，可提高 E2 检测的灵敏度，并可用于高准确度和可靠性地检测牛奶样品中的 E2。
