Nanulescu M, Rus H G, Niculescu F, Cristea A, Florescu P
Med Interne. 1987 Apr-Jun;25(2):99-104.
Kidney biopsies were obtained in 28 children with glomerular diseases and studied using indirect immunofluoreoscence and immunoperoxidase for the detection of IgG, IgA, IgM, Clq, C3c, C4, Fibrinogen and the neoantigens of the terminal C5b-9 complement complex. An affinity-purified rabbit IgG was used to recognize the neoantigens of the assembled terminal components of the complement system into the C5b-9 complex. The immunohistochemical studies were correlated to the clinical data and laboratory investigations. Fourteen of the 28 patients presented specific C5b-9 glomerular deposits; they were also present at the tubular sites in 8 patients and at the vascular sites in 12 patients. An unfavourable evolution was observed for patients presenting C5b-9 deposits in contrast to those without such deposits, even of the same histopathological pattern of glomerulonephritis. The presence of C5b-9 complex at the site of glomerular injury suggests a pathological involvement of the "in situ" complement activation and could be a marker in the prognosis of the glomerular diseases.
对28例肾小球疾病患儿进行肾活检，采用间接免疫荧光法和免疫过氧化物酶法检测IgG、IgA、IgM、Clq、C3c、C4、纤维蛋白原以及终末C5b - 9补体复合物的新抗原。用亲和纯化的兔IgG识别补体系统组装的终末成分进入C5b - 9复合物的新抗原。免疫组织化学研究与临床数据和实验室检查相关。28例患者中有14例出现特异性C5b - 9肾小球沉积物；8例患者的肾小管部位以及12例患者的血管部位也有沉积物。与无此类沉积物的患者相比，出现C5b - 9沉积物的患者病情进展不利，即使是具有相同组织病理学模式的肾小球肾炎患者也是如此。肾小球损伤部位存在C5b - 9复合物提示“原位”补体激活的病理参与，可能是肾小球疾病预后的一个标志物。
