Unidad Colaborativa de Bioensayos Entomológicos (UCBE) y del Laboratorio de Control Biológico (LCB) para Ae. aegypti, Universidad Autónoma de Yucatán (UADY), Campus de Ciencias Biológicas y Agropecuarias, Mérida, Yucatán, Mexico.
Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Universidad Autonoma de Yucatán (UADY), Merida, Yucatan, Mexico.
J Insect Sci. 2020 Sep 1;20(5). doi: 10.1093/jisesa/ieaa096.
This study reports the results of a molecular screening for Wolbachia (Wb) infection in Aedes albopictus (Skuse) populations recently established in the Yucatan Peninsula, Mexico. To do so, collections of free-flying adults with BG traps and emerged adults from eggs after ovitrap field collections were performed in three suburban localities of the city of Merida, Yucatan. Overall, local populations of Ae. albopictus present a natural Wb infection rate of ~40% (18 of 45). Wb infection was detected in both field-collected adults (76.5%, 13 of 17) and eggs reared (17.8%, 5 of 28) and in 37.9% (11/29) of females and 43.7% (7/16) of male Ae. albopictus mosquitoes. An initial screening for Wolbachia strain typing showed that native Ae. albopictus were naturally coinfected with both wAlbA and wAlbB strains. The knowledge of the prevalence and diversity of Wolbachia strains in local populations of Aedes mosquitoes is part of the baseline information required for current and future Wolbachia-based vector control approaches to be conducted in Mexico.
本研究报告了在墨西哥尤卡坦半岛最近建立的白纹伊蚊（Aedes albopictus）种群中沃尔巴克氏体（Wolbachia）感染的分子筛查结果。为此，在尤卡坦州梅里达市的三个郊区地点，使用 BG 诱捕器收集自由飞行的成虫和从卵中孵化的成虫。总体而言，当地的白纹伊蚊种群自然感染沃尔巴克氏体的比例约为 40%（45 只中有 18 只）。在野外采集的成虫（76.5%，17 只中有 13 只）和饲养的卵（17.8%，28 只中有 5 只）中均检测到了沃尔巴克氏体感染，37.9%（29 只中有 11 只）的雌性和 43.7%（16 只中有 7 只）的雄性白纹伊蚊也受到了感染。沃尔巴克氏体菌株分型的初步筛选表明，本地白纹伊蚊自然感染了 wAlbA 和 wAlbB 两种菌株。了解当地伊蚊种群中沃尔巴克氏体菌株的流行情况和多样性是在墨西哥开展基于沃尔巴克氏体的蚊虫控制方法所需的基线信息的一部分。
