Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China.
Jianyang Animal Disease Prevention and Control Center of Sichuan Province, Jianyang 641400, China.
Infect Genet Evol. 2020 Dec;86:104585. doi: 10.1016/j.meegid.2020.104585. Epub 2020 Oct 6.
Innate immunity is the first line against the invasion of pathogenic microorganisms. Over the past several years, the antiviral activity and mechanisms of the IFIT5 gene have been confirmed in mammals. However, more information is needed on the role of IFIT5 in response to viral infection in chickens. In this study, we examined the mRNA expression profile of chicken IFIT5 (chIFIT5) in different tissues and explored how chIFIT5 transduces upstream signaling to the downstream adaptor. Relative mRNA expression level of chIFIT5 was the highest in spleen and expression level of chIFIT5 was significantly up-regulated following Newcastle disease virus (NDV) infection, and polyinosinic:polycytidylic acid [poly (I:C)]- and poly(deoxyadenylic-thymidylic) [poly (dA:dT)]-triggered antiviral immune responses. Chicken MDA5, MAVS, and IRF7 positively regulated the mRNA expression of chIFIT5. Overexpression of chIFIT5 could promote IRF7- and NF-κB-mediated gene expression following NDV infection or transfection with poly (I:C). These results suggested that chIFIT5 is an important enhancer of the innate immunity response.
先天免疫是抵御病原微生物入侵的第一道防线。在过去的几年中，已经在哺乳动物中证实了 IFIT5 基因的抗病毒活性和机制。然而，我们需要更多关于 IFIT5 在鸡对病毒感染的反应中的作用的信息。在这项研究中，我们检查了不同组织中鸡 IFIT5（chIFIT5）的 mRNA 表达谱，并探讨了 chIFIT5 如何将上游信号转导到下游衔接蛋白。chIFIT5 的相对 mRNA 表达水平在脾脏中最高，并且在新城疫病毒（NDV）感染后表达水平显著上调，以及多聚肌苷酸：多聚胞苷酸[poly（I:C）]和聚（脱氧腺苷-胸腺嘧啶）[poly（dA:dT）]触发抗病毒免疫反应。鸡 MDA5、MAVS 和 IRF7 正向调节 chIFIT5 的 mRNA 表达。chIFIT5 的过表达可促进 NDV 感染或 poly（I:C）转染后 IRF7 和 NF-κB 介导的基因表达。这些结果表明 chIFIT5 是先天免疫反应的重要增强子。
