Wang Ying, Duan Meng, Rahman Mahbubur, Yang Ming, Zhao Weixin, Zhou Shukui, Gao Guo, Fu Qiang
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Eastern Institute of Urologic Reconstruction, Shanghai Jiao Tong University, Shanghai 200233, China.
Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Acta Biomater. 2020 Nov;117:156-166. doi: 10.1016/j.actbio.2020.09.049. Epub 2020 Oct 6.
Injection of urethral bulking agents is a low-risk, minimally invasive surgical procedure to treat stress urinary incontinence (SUI). In this study, we developed a promising injectable bulking agent comprising extracellular matrix fragments of adipose-derived stem cell sheets (ADSC ECM) and investigated its effectiveness in urethral bulking therapy. The structural integrity and proteins of ADSC sheet ECM were well retained in decellularized ADSC ECM fragments. To locate transplanted ADSC ECM fragments, they were labeled with ultrasmall super-paramagnetic iron oxide nanoparticles, which enabled in vivo monitoring after implantation in a SUI rat model for up to 4 weeks. When ADSC ECM fragments were injected into the rat urethra, they became fully integrated with the surrounding tissue within 1 week. Four weeks after transplantation, host cells had regenerated within the ADSC ECM fragment injection area. Moreover, new smooth muscle tissue had formed around the ADSC ECM fragments, as confirmed by positive staining of myosin. These results indicate that injection of ECM fragments may be a promising minimally invasive approach for treating SUI.
注射尿道填充剂是一种治疗压力性尿失禁（SUI）的低风险、微创外科手术。在本研究中，我们研发了一种有前景的可注射填充剂，其由脂肪来源干细胞片（ADSC）的细胞外基质片段（ADSC ECM）组成，并研究了其在尿道填充治疗中的有效性。ADSC片ECM的结构完整性和蛋白质在脱细胞的ADSC ECM片段中得到了很好的保留。为了定位移植的ADSC ECM片段，用超小超顺磁性氧化铁纳米颗粒对其进行标记，这使得在植入SUI大鼠模型后长达4周的时间内都能进行体内监测。当将ADSC ECM片段注射到大鼠尿道中时，它们在1周内与周围组织完全整合。移植后4周，宿主细胞在ADSC ECM片段注射区域内再生。此外，如肌球蛋白阳性染色所证实的，在ADSC ECM片段周围形成了新的平滑肌组织。这些结果表明，注射ECM片段可能是一种治疗SUI的有前景的微创方法。
