Laboratory of Biomaterials, Department of Chemical Engineering, Faculty of Engineering, University of Concepción, Concepción, Chile.
Laboratory of Biomaterials, Department of Chemical Engineering, Faculty of Engineering, University of Concepción, Concepción, Chile.
Colloids Surf B Biointerfaces. 2021 Jan;197:111398. doi: 10.1016/j.colsurfb.2020.111398. Epub 2020 Oct 4.
The natural mechanisms of the body cannot control massive hemorrhaging, resulting in a requirement for hemostatic intervention. In this study, Graphene oxide and Chitosan aerogels reinforced with grape seed (SD) and skin (SK) extracts were developed for use as hemostatic agents by evaluating the influence of pH on their synthesis, and the amount of grape extract added on the physical and chemical properties of the aerogels. The material was evaluated by FTIR, XRD, Raman spectroscopy, DLS, uniaxial compression tests and SEM. The capacity of the aerogels to absorb water, PBS and blood, as well as their coagulation capacity, were determined. In addition, the release profile for grape extracts in PBS and the material's cytotoxicity were determined. The aerogels that were synthesized under basic conditions and loaded with grape extracts were more rigid and negatively charged, and they presented smaller pores than the un-loaded acidic aerogels. For all aerogels, the hemoglobin absorption was greater than 90 % in the first 30 s. A higher density of adsorbed blood cells was observed on aerogels loaded with a higher amount of grape extract. The maximum release of extract from the aerogels occurred for those loaded with SK extracts at a basic pH; the aerogels that were prepared under acidic conditions dissolved in the media. Aerogels loaded with SK extracts under alkaline conditions were not cytotoxic toward human dermal fibroblasts and exhibited cell viabilities above 90 %. These findings suggest that these aerogels have the potential for use as hemostatic agents in wound management.
机体的自然止血机制无法控制大量出血，因此需要进行止血干预。在这项研究中，通过评估 pH 值对合成的影响以及添加的葡萄提取物量对水凝胶物理化学性质的影响，开发了氧化石墨烯和壳聚糖气凝胶，并用葡萄籽（SD）和葡萄皮（SK）提取物进行强化，用作止血剂。采用傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、拉曼光谱、动态光散射（DLS）、单轴压缩试验和扫描电子显微镜（SEM）对材料进行了评估。测定了水凝胶的吸水、PBS 和血液能力以及凝血能力。此外，还测定了 PBS 中葡萄提取物的释放曲线和材料的细胞毒性。在碱性条件下合成并负载葡萄提取物的气凝胶更硬且带负电荷，并且比未负载的酸性气凝胶具有更小的孔。对于所有气凝胶，在最初的 30 秒内血红蛋白吸收率均大于 90%。在负载较高量葡萄提取物的气凝胶上观察到吸附的血细胞密度更高。在碱性 pH 值下，负载 SK 提取物的气凝胶中提取物的最大释放量最高；在酸性条件下制备的气凝胶会在介质中溶解。在碱性条件下负载 SK 提取物的气凝胶对人皮肤成纤维细胞没有细胞毒性，细胞活力高于 90%。这些结果表明，这些气凝胶有可能用于伤口管理中的止血剂。
