Laboratory of Biomaterials, Department of Chemical Engineering, Faculty of Engineering, Universidad de Concepción, Concepción, Chile.
Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Concepción, Chile.
Colloids Surf B Biointerfaces. 2021 Oct;206:111941. doi: 10.1016/j.colsurfb.2021.111941. Epub 2021 Jun 25.
Aerogels based on gelatin and graphene oxide (GO) were synthesized by microwave-assisted reactions, incorporating grape skin extracts -high in proanthocyanidins (PAs)- to develop a hemostatic device with improved properties. The effects of incorporating PAs into the aerogels were investigated in relation to their physicochemical properties, absorption ability, clotting activity and cytotoxicity in human dermal fibroblast (HDF) cells. The aerogels presented highly resistant porous structures, capable of absorbing more than 50 times their weight when in contact with a phosphate saline solution (PBS) and fresh human blood. Interestingly, the addition of PAs increased the negative surface charges and the blood absorption ability of the aerogels, which may make them suitable for hemostasis. The incorporation of 5% and 10% (w/w) of extracts into the aerogels increased the total coagulated blood content by 36.6% and 24.5% compared with gelatin-GO aerogel, respectively. These improvements in the hemostatic properties of the aerogels were greater with the inclusion of 5% (w/w) of grape skin extracts into the aerogels. The aerogels were also able to adhere red blood cells onto their surfaces, which could favor the formation of stable fibrin networks to promote hemostasis. Their clotting activity suggested the activation of alternative routes based on complement coagulation systems. Finally, the aerogels were non-toxic for HDF cells and the PAs were successfully released from their matrices. Thus, gelatin-GO aerogels reinforced with PAs are promising as topical phytodrug delivery systems, with great potential for wound healing processes.
基于明胶和氧化石墨烯（GO）的气凝胶是通过微波辅助反应合成的，其中加入了富含原花青素（PAs）的葡萄皮提取物，以开发出具有改进性能的止血装置。研究了将 PAs 掺入气凝胶中对其物理化学性质、吸收能力、凝血活性和人真皮成纤维细胞（HDF）细胞的细胞毒性的影响。气凝胶具有高度耐多孔结构，当与磷酸盐生理盐水（PBS）和新鲜人血接触时，能够吸收超过其重量 50 倍的物质。有趣的是，添加 PAs 增加了气凝胶的负表面电荷和血液吸收能力，这可能使它们适用于止血。与明胶-GO 气凝胶相比，将 5%（w/w）和 10%（w/w）的提取物掺入气凝胶中，分别将总凝血含量提高了 36.6%和 24.5%。将 5%（w/w）的葡萄皮提取物掺入气凝胶中，可更大程度地改善气凝胶的止血性能。气凝胶还能够将红细胞粘附在其表面上，这有利于形成稳定的纤维蛋白网络，以促进止血。它们的凝血活性表明基于补体凝血系统的替代途径被激活。最后，气凝胶对 HDF 细胞无毒，并且 PAs 从其基质中成功释放。因此，用 PAs 增强的明胶-GO 气凝胶有望作为局部植物药递送系统，在伤口愈合过程中有很大的潜力。
