Ghazavi Atefeh, González-González Maria A, Romero-Ortega Mario I, Cogan Stuart F
Department of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080, USA.
Department of Biomedical Engineering, University of Houston, Houston, TX, 77204, USA.
Biosens Bioelectron. 2020 Dec 15;170:112608. doi: 10.1016/j.bios.2020.112608. Epub 2020 Sep 22.
Selective interfacing to small multifunctional nerves such as the vagus nerve (VN) which is the main multimodal autonomic nerve that provides a major communication pathway from vital peripheral organs to the brain, can have significant potential in treating and diagnosing diseases as well as enhancing our understanding of peripheral nerve circuits. Here we describe the fabrication of a 16-channel intraneural electrode array with ultramicro-dimensioned electrodes to achieve improved functionally selective recording. We demonstrate that the amorphous silicon carbide ultramicroelectrode arrays (a-SiC UMEAs) provide selectivity in the detection of neural activity in the cVN related to changes in systemic oxygenation and blood pressure. We will also demonstrate spatially selective recording of micro-compound action potentials (μCAPs) by electrical stimulation of the subdiaphragmatic branches of the VN. Distinct neural activity was recorded on electrodes separated by less than about 100 μm. This is the first time that this level of spatially selectivity recording has been demonstrated in the cVN with an intraneural multielectrode array.
与小型多功能神经（如迷走神经（VN））进行选择性连接具有巨大潜力，迷走神经是主要的多模式自主神经，为从重要外周器官到大脑的主要通信通路。这在疾病治疗和诊断以及增进我们对周围神经回路的理解方面都有重要意义。在此，我们描述了一种具有超微尺寸电极的16通道神经内电极阵列的制造方法，以实现功能选择性记录的改进。我们证明非晶硅碳超微电极阵列（a-SiC UMEAs）在检测与全身氧合和血压变化相关的迷走神经中枢（cVN）神经活动时具有选择性。我们还将通过电刺激膈下迷走神经分支来展示微复合动作电位（μCAPs）的空间选择性记录。在间距小于约100μm的电极上记录到了不同的神经活动。这是首次使用神经内多电极阵列在迷走神经中枢展示出这种水平的空间选择性记录。
