Natural Product Research Laboratory, Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 111 Joensuu, Finland.
School of Forest Sciences, University of Eastern Finland, P.O. Box 111 Joensuu, Finland.
Molecules. 2020 Oct 6;25(19):4558. doi: 10.3390/molecules25194558.
Knowledge about the defensive chemistry of coniferous trees has increased in recent years regarding a number of alkaloid compounds; in addition to phenolics and terpenes. Here, we show that Norway spruce ( (L.) H. Karst.), an important boreal zone tree species; accumulates 1,6-dehydropinidine (2-methyl-6-(2-propenyl)-1,6-piperideine) in its needles and bark. We reanalyzed previously published GC-MS data to obtain a full picture of 1,6-dehydropinidine in . 1,6-dehydropinidine appeared to especially accumulate in developing spring shoots. We used solid-phase partitioning to collect the alkaloid fraction of the sprouts and thin-layer chromatography to purify 1,6-dehydropinidine. The antibacterial properties of the 1,6-dehydropinidine fraction were tested using a broth microdilution method; with subsp. as a model organism. Based on our results 1,6-dehydropinidine is common in alkaloid extractions from (0.4 ± 0.03 mg g dw in mature needles) and it is especially abundant in young spruce shoots (2.7 ± 0.5 mg g dw). Moreover; 1,6-dehydropinidine extracted from sprouts showed mild antibacterial potential against subsp. (MIC 55 µg mL). The antibacterial activity of a plant compound thought of as an intermediate rather than an end-product of biosynthesis calls for more detailed studies regarding the biological function of these coniferous alkaloids.
近年来，人们对针叶树的防御化学物质有了更多的了解，包括许多生物碱化合物；除了酚类和萜类化合物。在这里，我们表明，挪威云杉（(L.) H. Karst.），一种重要的北方树种；在其针叶和树皮中积累了 1,6-去氢哌啶（2-甲基-6-(2-丙烯基）-1,6-哌啶）。我们重新分析了之前发表的 GC-MS 数据，以全面了解（.）中 1,6-去氢哌啶的情况。1,6-去氢哌啶似乎特别在发育中的春梢中积累。我们使用固相分配法收集芽的生物碱部分，并使用薄层色谱法纯化 1,6-去氢哌啶。使用肉汤微量稀释法测试了 1,6-去氢哌啶部分的抗菌特性；以 subsp. 作为模式生物。根据我们的结果，1,6-去氢哌啶在从（成熟针叶中 0.4 ± 0.03 mg g dw）中提取的生物碱中很常见，并且在年轻的云杉芽中特别丰富（2.7 ± 0.5 mg g dw）。此外；从云杉芽中提取的 1,6-去氢哌啶对 subsp. （MIC 55 µg mL）表现出轻微的抗菌潜力。这种被认为是生物合成中间产物而不是最终产物的植物化合物的抗菌活性需要对这些针叶树生物碱的生物学功能进行更详细的研究。
