Bonilla-Lemus Patricia, Rojas-Hernández Saúl, Ramírez-Flores Elizabeth, Castillo-Ramírez Diego A, Monsalvo-Reyes Alejandro Cruz, Ramírez-Flores Miguel A, Barrón-Graciano Karla, Reyes-Batlle María, Lorenzo-Morales Jacob, Carrasco-Yépez María Maricela
Laboratorio de Microbiología Ambiental, Grupo CyMA, UIICSE, FES Iztacala, UNAM. Av. De Los Barrios 1, Los Reyes Iztacala, Tlalnepantla de Baz 54090, Estado de Mexico, Mexico.
Laboratorio de Inmunobiología Molecular y Celular, Sección de Estudios de Posgrado e Investigación. Escuela Superior de Medicina, Instituto Politécnico Nacional, Salvador Díaz Mirón esq. Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de Mexico 11340, CDMX, Mexico.
Pathogens. 2020 Oct 7;9(10):820. doi: 10.3390/pathogens9100820.
Members of the genus are free-living amoebae that are widely distributed in water and soil environments. Moreover, is a pathogenic amoeba species that causes a fatal disease in the central nervous system known as primary amoebic meningoencephalitis (PAM) in humans. Since most reported infections due to are reported in recreational waters worldwide, this study was aimed to describe the presence of these amoebic genus in Mexicali Valley irrigation channels of recreational use. A total of nine water samples were collected and processed by triplicate, in nine different sites of the Valley. After filtering and culturing the samples, plates were examined, and the observed amoebae were morphologically identified at the genus level. In addition, the pathogenicity of these amoebic isolates was checked, and molecular characterization was performed by PCR/sequencing. The results revealed the presence of spp. in all the channels sampled. Finally, molecular identification confirmed the presence of five different species of and . The presence of these protists, particularly , should be considered as a potential human health risk in the region.
该属成员为自由生活的变形虫，广泛分布于水和土壤环境中。此外，[具体物种名称未给出]是一种致病性变形虫物种，可在人类中枢神经系统引发一种致命疾病，即原发性阿米巴脑膜脑炎（PAM）。由于全球范围内大多数因[具体物种名称未给出]导致的感染病例都报告于娱乐用水中，本研究旨在描述这些变形虫属在墨西卡利谷娱乐用灌溉渠道中的存在情况。在该谷地的九个不同地点共采集了九个水样，并进行了三次重复处理。对样本进行过滤和培养后，检查平板，并在属水平上对观察到的变形虫进行形态学鉴定。此外，还检查了这些变形虫分离株的致病性，并通过PCR/测序进行分子特征分析。结果显示在所有采样渠道中均存在[具体物种名称未给出]属。最后，分子鉴定证实存在五种不同的[具体物种名称未给出]属和[另一具体物种名称未给出]属物种。这些原生生物的存在，尤其是[具体物种名称未给出]，应被视为该地区潜在的人类健康风险。
