Warren J R, Kowalski M M, Wallas C H
Infect Immun. 1977 Aug;17(2):389-94. doi: 10.1128/iai.17.2.389-394.1977.
The adsorption to human erythrocytes of Escherichia coli lipopolysaccharide treated by mild alkaline hydrolysis (h-LPS) stimulated an increase in the intracellular Na+ concentration and a decrease in the intracellular K+ concentration of the erythrocytes. Erythrocytes treated by h-LPS remained responsive to the membrane adenosine triphosphatase inhibitors ouabain and ethacrynic acid, indicating that hLPS did not alter erythrocyte cations be depleting energy intermediates or uncoupling energy metabolism from active cation transport. The h-LPS-treated erythrocytes became non-agglutinable by the lectin concanavalin A prior to the development of changes in intracellular cations. In addition, h-LPS-treated erythrocytes demonstrated a three-fold greater cation response to ethacrynic acid than the untreated erythrocytes; this greater response was probably due to local membrane effects by h-LPS on the ethacrynic acid-sensitive adenosine triphosphatase. It is suggested that the h-LPS-induced alteration of erythrocyte cation content was secondary to an increase in ion permeability localized to the concanavalin A receptor regions of the erythrocyte membrane, possibly combined with indirect effects of membrane-bound h-LPS on ethacrynic acid-sensitive adenosine triphosphatase.
经轻度碱水解处理的大肠杆菌脂多糖（h-LPS）对人红细胞的吸附作用，刺激红细胞内Na⁺浓度升高，K⁺浓度降低。经h-LPS处理的红细胞对膜三磷酸腺苷酶抑制剂哇巴因和依他尼酸仍有反应，这表明h-LPS不会通过消耗能量中间体或使能量代谢与主动阳离子转运解偶联来改变红细胞阳离子。在细胞内阳离子发生变化之前，经h-LPS处理的红细胞就不再能被凝集素伴刀豆球蛋白A凝集。此外，经h-LPS处理的红细胞对依他尼酸的阳离子反应比未处理的红细胞大两倍；这种更大的反应可能是由于h-LPS对依他尼酸敏感的三磷酸腺苷酶产生局部膜效应所致。有人认为，h-LPS诱导的红细胞阳离子含量改变是继发于红细胞膜伴刀豆球蛋白A受体区域离子通透性增加，可能还伴有膜结合的h-LPS对依他尼酸敏感的三磷酸腺苷酶的间接作用。
