鉴定 Hsa_circ_0104824 作为乳腺癌的潜在生物标志物。
Identification of Hsa_circ_0104824 as a Potential Biomarkers for Breast Cancer.
机构信息
105002The Clinical Medical School of Ningxia Medical University, Yinchuan, China.
105002The General Hospital of Ningxia Medical University, Yinchuan, China.
出版信息
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820960745. doi: 10.1177/1533033820960745.
BACKGROUND
To study the specific expression of circular RNA (circRNA) in breast cancer and adjacent normal tissues to identify differentially expressed circRNA in breast cancer patients. To study the correlation between circRNA and clinical data of breast cancer, and to evaluate its potential as a breast cancer biomarker.
METHODS
The differential expression of circRNAs between the breast cancer tissues and adjacent normal tissues was screened by Human CircRNA Microarray. Candidate circRNAs verified by qRT-PCR. CircRNA was analyzed by Agilent GeneSpring 13.0 software. SPSS23.0, GraphPad Prism, and Sigmaplot software were used for statistical analysis. Perform test, one-way ANOVA, curve regression analysis and ROC curve analysis for evaluating the diagnostic value of circular RNA.
RESULTS
Among the 2021 differentially expressed circRNAs, 546 were up-regulated and 1475 were down-regulated in breast cancer tissues. The validation study demonstrated that six circRNAs were downregulated. Among them, hsa_circ_0104824 proved high correlation between breast cancer tissues and plasma samples in its expression and diagnostic value.
CONCLUSION
Hsa_circ_0104824 may serve as a promising predictive biomarker and therapeutic target for patients with breast cancer.
背景
研究环状 RNA（circRNA）在乳腺癌及癌旁正常组织中的特异性表达，筛选乳腺癌患者差异表达的 circRNA。研究 circRNA 与乳腺癌临床资料的相关性，评估其作为乳腺癌生物标志物的潜力。
方法
采用 Human CircRNA Microarray 筛选乳腺癌组织与癌旁正常组织间差异表达的 circRNAs，qRT-PCR 验证候选 circRNAs。应用 Agilent GeneSpring 13.0 软件进行 circRNA 分析。采用 SPSS23.0、GraphPad Prism 和 Sigmaplot 软件进行统计学分析。采用 t 检验、单因素方差分析、曲线回归分析和 ROC 曲线分析评估环状 RNA 的诊断价值。
结果
在 2021 个差异表达的 circRNAs 中，乳腺癌组织中 546 个上调，1475 个下调。验证研究表明，有 6 个 circRNAs 下调，其中 hsa_circ_0104824 在表达和诊断价值方面与乳腺癌组织和血浆样本高度相关。
结论
hsa_circ_0104824 可能成为一种有前途的预测性生物标志物和治疗靶点，用于乳腺癌患者。
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