Department of Breast and Thyroid Surgery, Wuhan Integrated TCM & Western Medicine Hospital, Hubei, China.
J Clin Lab Anal. 2020 Sep;34(9):e23393. doi: 10.1002/jcla.23393. Epub 2020 Aug 18.
Cell-free circular RNAs (circRNAs) are stable and abundantly exist in body fluids. In this study, we aimed to investigate plasma cell-free circRNAs as a novel class of biomarkers for the diagnosis of breast cancer (BC).
Differentially expressed circRNAs from 6 normal and 6 BC plasma samples were detected by microarray. Hsa_circ_0008673 was then screened and validated in the plasma of 102 normal and 378 BC samples. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value. The correlations between hsa_circ_0008673 expression and demographic characteristics, tumor features, and prognosis were analyzed. The effects of hsa_circ_0008673 on BC cell proliferation and metastasis were also measured.
Of the top ten up-regulated (hsa_circ_0008673, hsa_circ_0008500, hsa_circ_0005260, hsa_circ_0003423, hsa_circ_0119881, hsa_circ_0000987, hsa_circ_0007386, hsa_circ_0000091, hsa_circ_0016601, and hsa_circ_0008549) and top ten down-regulated (hsa_circ_0000826, hsa_circ_0072697, hsa_circ_0004587, hsa_circ_0000471, hsa_circ_0007786, hsa_circ_0001417, hsa_circ_0005982, hsa_circ_0001566, hsa_circ_0003823, and hsa_circ_0003823) circRNAs from microarray, hsa_circ_0008673 was the most significantly up-regulated circRNA in BC, and represented a good diagnostic value. Hsa_circ_0008673 was remarkably down-regulated after breast mastectomy. Hsa_circ_0008673 expression was associated with larger tumor size, distant metastasis, positive estrogen receptor (ER) status, and positive progesterone receptor (PR) status. Additionally, hsa_circ_0008673 could serve as a prognostic predicator of overall survival (OS) and disease-specific survival (DSS). Cell assays proved that hsa_circ_0008673 knockdown contributed to inhibition of tumor cell proliferation and migration.
Plasma cell-free hsa_circ_0008673 was up-regulated in BC, which was associated with poorer prognosis and promoted tumor proliferation and metastasis. Hsa_circ_0008673 is a promising biomarker for tumor diagnosis and prognostic assessment of BC patients.
无细胞循环 RNA（circRNAs）在体液中稳定且大量存在。本研究旨在探讨血浆无细胞 circRNAs 是否可作为乳腺癌（BC）诊断的新型生物标志物。
采用微阵列检测 6 例正常和 6 例 BC 血浆样本中的差异表达 circRNAs。然后在 102 例正常和 378 例 BC 样本中筛选和验证 hsa_circ_0008673。使用受试者工作特征（ROC）曲线评估诊断价值。分析 hsa_circ_0008673 表达与人口统计学特征、肿瘤特征和预后的相关性。还测量了 hsa_circ_0008673 对 BC 细胞增殖和转移的影响。
在微阵列中，十大上调（hsa_circ_0008673、hsa_circ_0008500、hsa_circ_0005260、hsa_circ_0003423、hsa_circ_0119881、hsa_circ_0000987、hsa_circ_0007386、hsa_circ_0000091、hsa_circ_0016601 和 hsa_circ_0008549）和十大下调（hsa_circ_0000826、hsa_circ_0072697、hsa_circ_0004587、hsa_circ_0000471、hsa_circ_0007786、hsa_circ_0001417、hsa_circ_0005982、hsa_circ_0001566、hsa_circ_0003823 和 hsa_circ_0003823）circRNAs 中，hsa_circ_0008673 在 BC 中上调最显著，具有良好的诊断价值。hsa_circ_0008673 在乳房切除术后显著下调。hsa_circ_0008673 的表达与肿瘤体积较大、远处转移、雌激素受体（ER）阳性和孕激素受体（PR）阳性有关。此外，hsa_circ_0008673 可作为总生存（OS）和疾病特异性生存（DSS）的预后预测因子。细胞试验证明 hsa_circ_0008673 敲低有助于抑制肿瘤细胞增殖和迁移。
BC 患者血浆无细胞 hsa_circ_0008673 上调，与预后不良相关，并促进肿瘤增殖和转移。hsa_circ_0008673 是一种有前途的肿瘤诊断和 BC 患者预后评估的生物标志物。
