Department of Crop Science, Chungnam National University, Yuseong-gu, Korea.
Division of Life Sciences and Bio-Resource and Environmental Center, Incheon National University, Yeonsu-gu, Korea.
Prep Biochem Biotechnol. 2021;51(5):467-474. doi: 10.1080/10826068.2020.1830415. Epub 2020 Oct 12.
The dried root of is a well-known herbal medicine, and it is useful in treating chronic diseases and weakness, as well as for improving overall health and vitality. Astragalosides, which are root quality indicators of , are natural triterpenoid saponins that are used in the treatment of diabetes and cardiovascular diseases. Currently, there is an urgent need to improve their production because of their low quantity in plants and the difficulty of chemical synthesis. In this study, yeast extract was added to facilitate elicitation in -mediated hairy root cultures, thereby enhancing astragaloside production in . Results showed that yeast extract could stimulate astragaloside content effectively in the hairy roots of . Moreover, astragaloside accumulation was positively correlated with the upregulation of mevalonate biosynthetic gene expression in the presence of yeast extract. Our study demonstrated that pretreatment with yeast extract (3.65 mM) for 72 h serves as an effective strategy to enhance astragaloside levels in hairy root cultures. Thus, these optimal conditions can provide valuable information for the improvement of astragaloside industrial production in .
是一种著名的草药，其干根可用于治疗慢性病和虚弱症，也有助于改善整体健康和活力。黄芪甲苷是黄芪的根质量指标，是一种天然三萜皂苷，用于治疗糖尿病和心血管疾病。由于其在植物中的含量低且化学合成困难，目前迫切需要提高其产量。在这项研究中，添加酵母提取物以促进介导的发根培养物中的诱导，从而提高黄芪甲苷的产量。结果表明，酵母提取物可以有效地刺激黄芪甲苷在发根中的含量。此外，在存在酵母提取物的情况下，黄芪甲苷的积累与甲羟戊酸生物合成基因表达的上调呈正相关。我们的研究表明，用酵母提取物（3.65mM）预处理 72h 是提高黄芪毛状根培养物中黄芪甲苷水平的有效策略。因此，这些最佳条件可为提高黄芪甲苷的工业生产提供有价值的信息。
