Petrova Maria, Geneva Maria, Trendafilova Antoaneta, Miladinova-Georgieva Kamelia, Dimitrova Lyudmila, Sichanova Mariana, Nikolova Milena, Ivanova Viktoria, Dimitrova Margarita, Sozoniuk Magdalena
Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, Bulgaria.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 9, 1113 Sofia, Bulgaria.
Plants (Basel). 2025 Mar 19;14(6):967. doi: 10.3390/plants14060967.
L. is an important herbal medicinal plant that belongs to the family Asteraceae. This plant has been known for its medicinal uses for centuries. exhibits several pharmacological properties, including immunomodulatory, anti-inflammatory, anticancer, antioxidant, and antibacterial effects. For the first time, the impacts of the biotic elicitor yeast extract, and the abiotic elicitor salicylic acid on micropropagation, antioxidant potential, and accumulation of caffeoylquinic acids in arnica in vitro shoots were assessed. The results showed that yeast extract applied at 100 mg/L significantly promotes shoot multiplication, biomass yield, total phenolic content, and synthesis of caffeoylquinic acids compared to control untreated shoots. Flavonoid content was the highest in samples treated with 200 mg/L of yeast extract, although at this concentration the measured biometric parameters began to decrease. Salicylic acid at 100 µM was found to be effective in the induction of vigorous shoots, shoot height growth, and biomass accumulation; nevertheless, this elicitor downregulated the caffeoylquinic acid level, total phenolics, and flavonoids. Increasing the concentration of salicylic acid to 200 µM caused shoot multiplication and fresh biomass accumulation reduction. Both elicitors modulated the activity of antioxidant enzymes against oxidative stress. Overall, the use of these substances can improve the growth and biomass yield in in vitro shoots.
山金车是一种重要的草药植物，属于菊科。几个世纪以来，这种植物一直以其药用价值而闻名。它具有多种药理特性，包括免疫调节、抗炎、抗癌、抗氧化和抗菌作用。首次评估了生物诱导剂酵母提取物和非生物诱导剂水杨酸对山金车离体芽微繁殖、抗氧化潜力和咖啡酰奎宁酸积累的影响。结果表明，与未处理的对照芽相比，以100 mg/L施用酵母提取物可显著促进芽的增殖、生物量产量、总酚含量以及咖啡酰奎宁酸的合成。在以200 mg/L酵母提取物处理的样品中，类黄酮含量最高，尽管在此浓度下测得的生物特征参数开始下降。发现100 µM的水杨酸可有效诱导健壮芽、芽高生长和生物量积累；然而，这种诱导剂下调了咖啡酰奎宁酸水平、总酚和类黄酮。将水杨酸浓度提高到200 µM会导致芽增殖和新鲜生物量积累减少。两种诱导剂都调节了抗氧化酶对抗氧化应激的活性。总体而言，使用这些物质可以提高山金车离体芽的生长和生物量产量。
