胰岛素、血清和地塞米松对人淋巴母细胞系IM-9中胰岛素受体mRNA水平的影响。

The effect of insulin, serum and dexamethasone on mRNA levels for the insulin receptor in the human lymphoblastoic cell line IM-9.

作者信息

Maassen J A, Krans H M, Möller W

出版信息

Biochim Biophys Acta. 1987 Aug 19;930(1):72-8. doi: 10.1016/0167-4889(87)90157-1.

DOI:10.1016/0167-4889(87)90157-1

Abstract

The effect of insulin, serum and dexamethasone on mRNA levels in the insulin receptor in the human lymphoblastoic cell line IM-9 was examined. To this end, mRNA levels were quantitated by Northern blot analysis using a labeled cDNA probe for the insulin receptor. The presence of 0.1 microM dexamethasone in the medium had a strong stimulatory effect on mRNA levels in insulin receptor, suggesting the presence of a glucocorticoid inducible enhancer element near the insulin receptor gene. Also, the nature of the serum had an effect on insulin receptor mRNA levels, as cells maintained in 10% fetal calf serum had insulin receptor mRNA levels that were 40-50% of those found in IM-9 cells maintained in 1% newborn serum. Variations in insulin receptor mRNA levels led in each situation to concordant variations in insulin binding. Insulin levels of up to 1 microM had no effect on hybridizable insulin receptor mRNA levels making an insulin-induced feed-back mechanism on gene expression or mRNA stability unlikely.

摘要

研究了胰岛素、血清和地塞米松对人淋巴母细胞系IM-9中胰岛素受体mRNA水平的影响。为此，使用胰岛素受体的标记cDNA探针通过Northern印迹分析对mRNA水平进行定量。培养基中存在0.1微摩尔地塞米松对胰岛素受体的mRNA水平有强烈的刺激作用，这表明在胰岛素受体基因附近存在糖皮质激素诱导增强子元件。此外，血清的性质对胰岛素受体mRNA水平有影响，因为在10%胎牛血清中培养的细胞的胰岛素受体mRNA水平是在1%新生血清中培养的IM-9细胞中发现的水平的40%-50%。在每种情况下，胰岛素受体mRNA水平的变化都会导致胰岛素结合的一致变化。高达1微摩尔的胰岛素水平对可杂交的胰岛素受体mRNA水平没有影响，这使得胰岛素诱导的基因表达或mRNA稳定性反馈机制不太可能存在。