Iwama N, Nomura M, Kajimoto Y, Imano E, Kubota M, Watarai T, Kawamori R, Shichiri M, Kamada T
First Department of Medicine, Osaka University Hospital, Japan.
Diabetologia. 1987 Nov;30(11):899-901. doi: 10.1007/BF00274801.
The effect of glucocorticoid (dexamethasone) on insulin receptor mRNA was examined in the IM-9 cell line by dot hybridization analysis using insulin receptor c-DNA probe (phINSR 13-1). Insulin receptor mRNA was found in a dose- and time-dependent manner, to increase during a 24-h culture with glucocorticoid. Although there has been some dispute about the effect of glucocorticoid on degradation of the insulin receptor itself, we have found glucocorticoid had no significant effect on the degradation rate of insulin receptor mRNA. These results suggest that glucocorticoid has the effect of increasing de novo synthesis of insulin receptor mRNA. But the molecular mechanism of glucocorticoid's effect still needs to be investigated.
通过使用胰岛素受体c-DNA探针（phINSR 13-1）进行点杂交分析，在IM-9细胞系中检测了糖皮质激素（地塞米松）对胰岛素受体mRNA的影响。发现胰岛素受体mRNA在与糖皮质激素进行24小时培养期间，呈剂量和时间依赖性增加。尽管关于糖皮质激素对胰岛素受体自身降解的影响存在一些争议，但我们发现糖皮质激素对胰岛素受体mRNA的降解速率没有显著影响。这些结果表明，糖皮质激素具有增加胰岛素受体mRNA从头合成的作用。但糖皮质激素作用的分子机制仍有待研究。
