Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Photodermatol Photoimmunol Photomed. 2021 Mar;37(2):115-122. doi: 10.1111/phpp.12618. Epub 2020 Oct 24.
Photodynamic antimicrobial chemotherapy (PACT) is a promising modality for eradication of microorganisms from the wound. This study aimed to investigate the effectiveness of PACT using indocyanine green (ICG) for reduction of bacterial load of oral ulcers in rats and its impact on the healing process.
In this experimental study, 50 adult male Sprague Dawley rats were recruited. Oral ulcers were surgically made on the left cheek mucosa, and animals were randomly assigned into five groups (n = 10). Wound site in groups 1, 2, and 3 was irrigated with the sterile saline (0.9%), chlorhexidine (CHX; 0.2%), and ICG solutions (1 mg/mL), respectively. Group 4 was exposed to laser irradiation using 810 nm diode laser on continuous-wave mode for 30 seconds (fluence: 55 J/cm , power: 300 mW, spot size: 4.5 mm). In group 5, PACT was performed using topical application of ICG followed by laser irradiation in the same way as the previous group. Bacterial load of oral ulcers was assessed before and after each treatment modality. Besides, rats were sacrificed on the 5th day post ulceration and histological features of healing were evaluated.
Bacterial load was significantly reduced merely in the PACT-ICG-treated group by one log (P < .0001). Animals in the PACT-ICG-treated group also showed an accelerated healing in comparison with others on the 5th day of an experiment.
Photodynamic antimicrobial chemotherapy using topical application of ICG has a potential to reduce the bacterial load of oral ulcers and accelerate wound repair. Therefore, it can be considered as an alternative to currently available modalities for wound management.
光动力抗菌化疗（PACT）是一种很有前途的方法，可以消除伤口中的微生物。本研究旨在探讨使用吲哚菁绿（ICG）减少大鼠口腔溃疡细菌负荷的效果及其对愈合过程的影响。
在这项实验研究中，招募了 50 只成年雄性 Sprague Dawley 大鼠。在左侧颊粘膜上进行口腔溃疡手术，将动物随机分为五组（n=10）。第 1、2 和 3 组的伤口部位分别用无菌生理盐水（0.9%）、洗必泰（CHX；0.2%）和 ICG 溶液（1mg/mL）冲洗。第 4 组使用 810nm 二极管激光在连续波模式下进行激光照射 30 秒（通量：55J/cm，功率：300mW，光斑尺寸：4.5mm）。第 5 组采用 ICG 局部应用后再进行激光照射的方式进行 PACT，方法与前一组相同。在每次治疗后评估口腔溃疡的细菌负荷。此外，大鼠在溃疡后第 5 天处死，并评估愈合的组织学特征。
仅在 PACT-ICG 治疗组中，细菌负荷显著降低了一个对数级（P<.0001）。与其他组相比，PACT-ICG 治疗组的动物在实验第 5 天的愈合速度也更快。
使用 ICG 局部应用的光动力抗菌化疗具有降低口腔溃疡细菌负荷和加速伤口修复的潜力。因此，它可以被认为是目前伤口管理方法的一种替代方法。
