College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Fujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR China.
Fujian Provincial Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
J Glob Antimicrob Resist. 2020 Dec;23:251-255. doi: 10.1016/j.jgar.2020.09.018. Epub 2020 Oct 9.
The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China.
A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 to 2016 in Southern China and were screened for the presence of the cfr gene by PCR. The cfr-carrying P. multocida strains were subjected to antimicrobial susceptibility testing, S1 nuclease PFGE and Southern blot hybridisation, conjugative transfer and analysis of genetic environment of the cfr gene.
Among 113 P. multocida isolates, strains FJ6671 and FJ6683 from Muscovy duck harboured the cfr gene and presented a multiresistant phenotype. The cfr gene in the two strains was located on an ∼40-kb conjugative plasmid in different genetic environments, including ISApl1-cfr-IS26 and IS26-cfr-IS256.
These results demonstrate plasmid-carried cfr in P. multocida and suggest that transposition and homologous recombination mediated by IS26, ISApl1 and IS256 might have played an important role in transfer of the cfr gene in P. multocida. To the best of our knowledge, this is the first report of the cfr gene in P. multocida. Active and ongoing surveillance of cfr in P. multocida is urgently warranted.
本研究旨在调查中国禽源多杀性巴氏杆菌中多耐药基因 cfr 基因的存在及其遗传环境。
从 2003 年至 2016 年，从中国南方患病家禽（鸭、鸡和鹅）中收集了 113 株多杀性巴氏杆菌分离株，并通过 PCR 筛选 cfr 基因的存在。对携带 cfr 基因的多杀性巴氏杆菌菌株进行药敏试验、S1 核酸酶 PFGE 和 Southern blot 杂交、接合转移以及 cfr 基因遗传环境分析。
在 113 株多杀性巴氏杆菌分离株中，来自麝香鸭的菌株 FJ6671 和 FJ6683 携带 cfr 基因并呈现多耐药表型。这两个菌株中的 cfr 基因位于不同遗传环境中的约 40kb 可接合质粒上，包括 ISApl1-cfr-IS26 和 IS26-cfr-IS256。
这些结果表明 cfr 基因存在于多杀性巴氏杆菌中，并提示 IS26、ISApl1 和 IS256 介导的转座和同源重组可能在多杀性巴氏杆菌 cfr 基因的转移中发挥了重要作用。据我们所知，这是多杀性巴氏杆菌中 cfr 基因的首次报道。迫切需要对多杀性巴氏杆菌中的 cfr 基因进行积极和持续的监测。
