一株携带 floR 质粒的多重耐药强毒型巴氏杆菌 ST342 菌株的出现。
Emergence of a multidrug-resistant hypervirulent Pasteurella multocida ST342 strain with a floR-carrying plasmid.
机构信息
Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
出版信息
J Glob Antimicrob Resist. 2020 Mar;20:348-350. doi: 10.1016/j.jgar.2019.09.012. Epub 2019 Sep 23.
OBJECTIVES
To date, very few hypervirulent and multiantibiotic-resistant bacterial strains have been reported. This study reports the first hypervirulent and multiantibiotic-resistant Pasteurella multocida sequence type 342 (ST342) strain (GH161213) isolated from a Pekin duck in China.
METHODS
Minimum inhibitory concentrations (MICs) were determined according to Clinical and Laboratory Standards Institute (CLSI) guidelines (VET01-A4, 2013). Determination of the P. multocida GH161213 median lethal dose (LD) was determined in a mouse model and in ducklings. Plasmid pRCAD0338PM-1 was transferred to Escherichia coli J53 by conjugation. The whole genome sequence of P. multocida GH161213 was obtained using an Illumina HiSeq 2500 system. Antimicrobial resistance genes were analysed using the Comprehensive Antibiotic Resistance Database (CARD).
RESULTS
Pasteurella multocida GH161213 is a hypervirulent strain with an LD of <10 CFU in a mouse model and in ducklings. It also has a high level of multidrug resistance. Strain GH161213 contains a small conjugative plasmid harbouring the floR florfenicol resistance gene. It also contains multiple other antimicrobial resistance mechanisms.
CONCLUSION
The genome sequence of P. multocida GH161213 reveals a multidrug-resistant genotype. This is the first reported hypervirulent and multiantibiotic-resistant P. multocida strain.
目的
迄今为止，报道的高毒力和多耐药细菌株非常少。本研究报告了首例从中国北京鸭中分离出的高毒力和多耐药巴氏杆菌 342 型（ST342）（GH161213）菌株。
方法
根据临床和实验室标准协会（CLSI）指南（VET01-A4，2013）测定最小抑菌浓度（MIC）。采用小鼠模型和雏鸭测定巴氏杆菌 GH161213 的半数致死剂量（LD）。通过接合将质粒 pRCAD0338PM-1 转移至大肠杆菌 J53。采用 Illumina HiSeq 2500 系统获得巴氏杆菌 GH161213 的全基因组序列。采用抗生素耐药综合数据库（CARD）分析抗生素耐药基因。
结果
巴氏杆菌 GH161213 是一株高毒力菌株，在小鼠模型和雏鸭中 LD<10 CFU。该菌株还具有高水平的多药耐药性。GH161213 菌株含有一个小的可接合质粒，携带 floR 氟苯尼考耐药基因。它还包含其他多种抗生素耐药机制。
结论
GH161213 巴氏杆菌的基因组序列揭示了一种多药耐药基因型。这是首例报道的高毒力和多耐药巴氏杆菌菌株。
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