Instituto Dermatológico de Jalisco "Dr. José Barba Rubio", Secretaría de Salud Jalisco, Zapopan, Jalisco, México.
Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
J Investig Med. 2021 Jan;69(1):41-46. doi: 10.1136/jim-2020-001414. Epub 2020 Oct 12.
Basal cell carcinoma (BCC) is the most common dermatological neoplasms in Caucasian populations. In Mexico, a prevalence of 3.9 per 1000 habitants is estimated. Recently, the macrophage migration inhibitory factor (MIF) has been related to different types of cancer. Therefore, this study aimed to investigate the genetic association of haplotypes of [-794(CATT)5-8/-173G>C]MIF gene polymorphisms and its soluble levels in BCC. A total of 360 individuals were recruited for the study, that is, 180 of the total amounts were patients with BCC histologically confirmed and the remaining 180 individuals were identified as control subjects (CS). Both polymorphisms were genotyped by PCR and PCR-RFLP (restriction fragment length polymorphism), and MIF serum levels were measured by ELISA kit. A borderline difference was found between the 55 genotype and the susceptibility to BCC (5.6% vs 1.7% in BCC and CS, respectively, OR=3.7 and p=0.04). Furthermore, the haplotype 7G showed a significant association with BCC (p=0.02, OR=1.99). Concerning MIF soluble levels, patients with BCC showed a media of 2.1 ng/mL and CS showed 4.4 ng/mL, the comparison between groups was significant (p<0.01). Our findings suggest that the 55 genotype and the haplotype 7G are associated with the susceptibility to BCC; furthermore, a significant difference was found between MIF soluble levels in both study groups.
基底细胞癌（BCC）是白种人群中最常见的皮肤肿瘤。在墨西哥，估计每 1000 名居民中有 3.9 例。最近，巨噬细胞移动抑制因子（MIF）与不同类型的癌症有关。因此，本研究旨在探讨 MIF 基因[-794（CATT）5-8/-173G>C]多态性单体型及其可溶性水平与 BCC 的遗传相关性。共招募了 360 名个体参与研究，其中 180 名总数量的患者经组织学证实患有 BCC，其余 180 名个体被确定为对照受试者（CS）。两种多态性均通过 PCR 和 PCR-RFLP（限制性片段长度多态性）进行基因分型，MIF 血清水平通过 ELISA 试剂盒进行测量。在 55 基因型与 BCC 的易感性之间发现了一个边界差异（BCC 中为 5.6%，CS 中为 1.7%，OR=3.7，p=0.04）。此外，7G 单倍型与 BCC 具有显著相关性（p=0.02，OR=1.99）。关于 MIF 可溶性水平，BCC 患者的平均值为 2.1ng/mL，CS 患者的平均值为 4.4ng/mL，两组之间的比较具有统计学意义（p<0.01）。我们的研究结果表明，55 基因型和 7G 单倍型与 BCC 的易感性相关；此外，两组之间 MIF 可溶性水平存在显著差异。
