Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Dermika Centro Dermatológico y Láser. Vallarta Nte, Guadalajara, Jalisco, Mexico.
J Clin Lab Anal. 2021 Nov;35(11):e23999. doi: 10.1002/jcla.23999. Epub 2021 Sep 17.
Macrophage inhibitory factor (MIF) is a pro-inflammatory cytokine secreted by several cells, including those in the immune system and the skin. The MIF gene contains the SNP -173 G> C and STR -794 CATT polymorphisms in the promoter region capable of affecting its activity. Our objective was to investigate the MIF polymorphisms as a risk factor for plaque psoriasis (PP) in the Mexican population.
We genotyped both MIF polymorphism (rs5844572 and rs755622) in 224 PP patients with a clinical and histopathological diagnosis and 232 control subjects (CS) by the PCR-RFLP method. MIF serum levels were determined by an ELISA kit.
We found significant differences in the genotypic and allelic frequencies for the MIF -173 G>C polymorphism; carriers of the GC genotype (OR 1.51, 95% CI 1.026-2.228, p = 0.03) and the C allele (OR 1.34, 95% CI 1.005-1.807, p = 0.04) had higher odds to present with PP. Moreover, the 6C haplotype was associated with PP risk (OR 2.10, 95% CI 1.22-3.69, p < 0.01). Also, the -173 CC genotype was associated with high MIF serum levels (p < 0.05).
The -173 GC genotype and the 6C haplotype of the MIF polymorphisms are associated with susceptibility to PP in the Mexican population.
巨噬细胞抑制因子（MIF）是一种由多种细胞分泌的促炎细胞因子，包括免疫系统和皮肤中的细胞。MIF 基因在启动子区域包含 SNP-173 G>C 和 STR-794 CATT 多态性，这些多态性能够影响其活性。我们的目的是研究 MIF 多态性作为墨西哥人群斑块状银屑病（PP）的风险因素。
我们通过 PCR-RFLP 方法对 224 例具有临床和组织病理学诊断的 PP 患者和 232 例对照（CS）进行了 MIF 多态性（rs5844572 和 rs755622）的基因分型。通过 ELISA 试剂盒测定 MIF 血清水平。
我们发现 MIF-173 G>C 多态性的基因型和等位基因频率存在显著差异；GC 基因型（OR 1.51，95%CI 1.026-2.228，p=0.03）和 C 等位基因（OR 1.34，95%CI 1.005-1.807，p=0.04）携带者发生 PP 的可能性更高。此外，6C 单倍型与 PP 风险相关（OR 2.10，95%CI 1.22-3.69，p<0.01）。此外，-173 CC 基因型与高 MIF 血清水平相关（p<0.05）。
MIF 多态性的-173 GC 基因型和 6C 单倍型与墨西哥人群中 PP 的易感性相关。
