Koc Kubra, Geyikoglu Fatime, Cakmak Ozge, Koca Aynur, Kutlu Zerrin, Aysin Ferhunde, Yilmaz Asli, Aşkın Hakan
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Naunyn Schmiedebergs Arch Pharmacol. 2021 Mar;394(3):469-479. doi: 10.1007/s00210-020-01984-1. Epub 2020 Oct 13.
This research is the first to use β-sitosterol on myocardial and renal tissues in renal ischemia/reperfusion (IR) damage. Female Wistar rats were randomly divided into three groups: control (sham), renal IR (50 min ischemia - 3 h reperfusion), and renal IR + 150 mg/kg/p.o. β-sitosterol (the rats were treated with β-sitosterol orally once 1 h before the IR procedure). β-Sitosterol pretreatment caused an increase in superoxide dismutase and glutathione activities and a decrease in malondialdehyde levels in the kidney and heart. Moreover, it alleviated histopathological changes and downregulated the levels of tumor necrosis factor-alpha and interleukin-6 and upregulated the levels of endothelial nitric oxide synthase. As conclusion, the potential of β-sitosterol for renal and cardiac necrosis and apoptosis appears to act by limiting inflammatory response and oxidative stress. Thus, the potential of this compound is noteworthy and may serve as a potential therapeutic in the treatment of acute organ damages due to renal IR.
本研究首次将β-谷甾醇用于肾缺血/再灌注（IR）损伤的心肌和肾组织。雌性Wistar大鼠随机分为三组：对照组（假手术组）、肾IR组（50分钟缺血 - 3小时再灌注）和肾IR + 150mg/kg/口服β-谷甾醇组（在IR手术前1小时给大鼠口服一次β-谷甾醇）。β-谷甾醇预处理使肾脏和心脏中的超氧化物歧化酶和谷胱甘肽活性增加，丙二醛水平降低。此外，它减轻了组织病理学变化，下调了肿瘤坏死因子-α和白细胞介素-6的水平，并上调了内皮型一氧化氮合酶的水平。结论是，β-谷甾醇对肾和心脏坏死及凋亡的潜在作用似乎是通过限制炎症反应和氧化应激来实现的。因此，这种化合物的潜力值得关注，可能作为治疗肾IR所致急性器官损伤的潜在疗法。
