Department of Chemistry, Aarhus University, Aarhus, 8000, Denmark.
iNano Interdisciplinary Nanoscience Centre, Aarhus University, Aarhus, 8000, Denmark.
Small. 2020 Nov;16(44):e2004280. doi: 10.1002/smll.202004280. Epub 2020 Oct 13.
Nanozymes are fundamentally interesting catalysts that are investigated as alternatives to fragile protein-enzymes for applications in biotechnology, for prodrug activation, and use in biomedicine, as well as the catalysts that contributed to the Origin of Life. However, until now, nanozymes mostly have been documented to exhibit activity as red/ox catalysts, whereas examples of activity outside this broad class of reactions are very few. Herein, activity of nanozymes on glucuronide prodrugs is investigated, specifically focusing on the mechanism of prodrug conversion reactions. The main finding of this work is that nanozymes exhibit glucuronide-like activity, but also catalyze prodrug conversion via esterase-like mechanism and facilitate group transfer reactions.
纳米酶是一类非常有趣的催化剂，它们被研究作为脆弱的蛋白质酶的替代品，用于生物技术、前药激活以及在生物医学中的应用，也被用于探究生命起源。然而，到目前为止，纳米酶主要被证明具有作为氧化还原催化剂的活性，而在这个广泛的反应类别之外的活性的例子非常少。在此，研究了纳米酶对葡萄糖醛酸苷前药的活性，特别关注前药转化反应的机制。这项工作的主要发现是纳米酶表现出类似葡萄糖醛酸苷的活性，但也通过酯酶样机制催化前药转化，并促进基团转移反应。
