Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi Avenue 71, 050038, Almaty, Kazakhstan.
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi Avenue 71, 050038, Almaty, Kazakhstan.
J Biotechnol. 2020 Dec 20;324:162-170. doi: 10.1016/j.jbiotec.2020.10.010. Epub 2020 Oct 10.
Current fresh water and energy shortage determines the need to study the possibilities of using living objects in bioenergy and environmental purification technologies. The development of waste-free technologies allows waste recycling, which saves raw materials and energy, in turn, reducing waste generation. The effect of different carbon dioxide concentrations and wastewater from households on the growth of cyanobacteria was studied in order to determine their capabilities in the purification processes. It was found that the optimal CO concentration for the cultivation of cyanobacteria Cyanobacterium sp. IPPAS B-1200 and Desertifilum sp. IPPAS B-1220 was 10 %, and for the Cyanobacterium aponinum IPPAS B-1201 - 5%. It was revealed that the cultivation of the cyanobacterium Cyanobacterium sp. IPPASB-1200 on wastewater from the water storage reduces the concentration of organic pollutants and, accordingly, improves the physicochemical properties of water. The cleaning percentage for selected pollutants was 68-100 %. It was shown that the most optimal ratio of wastewater to nutrient media for cyanobacteria cultivation were 25:75 and 50:50. The lipid content (%/dry weight) in the biomass of the studied strains of cyanobacteria ranges from 15 to 22% after cultivation in wastewater. It was determined that the strains of Cyanobacterium genus were the most suitable for the production of biodiesel according to their fatty acids composition. It was determined that lipids were composed of only saturated and monounsaturated fatty acids. As a result of the studies, the optimal conditions for the growth of Cyanobacterium sp. IPPAS B-1200 were determined. This microorganism has a good potential to produce biodiesel as a producer of saturated and monounsaturated middle-chain-length fatty acids.
目前淡水资源和能源短缺决定了有必要研究利用生物能源和环境净化技术中的活体的可能性。无废物技术的发展允许废物回收，从而节省了原材料和能源，进而减少了废物的产生。为了确定蓝藻在净化过程中的能力，研究了不同浓度的二氧化碳和家庭废水对蓝藻生长的影响。结果发现，蓝藻 Cyanobacterium sp. IPPAS B-1200 和 Desertifilum sp. IPPAS B-1220 的最佳 CO 浓度培养为 10%，而 Cyanobacterium aponinum IPPAS B-1201 的最佳 CO 浓度为 5%。结果表明，在储水的废水中培养蓝藻 Cyanobacterium sp. IPPASB-1200 可以降低有机污染物的浓度，从而改善水的物理化学性质。所选污染物的清洁率为 68-100%。结果表明，蓝藻培养的最佳废水与营养培养基的比例为 25:75 和 50:50。在废水培养后，研究的蓝藻菌株的生物质中的脂质含量（%/干重）范围为 15-22%。根据其脂肪酸组成，确定了 Cyanobacterium 属的菌株最适合生产生物柴油。结果表明，脂质仅由饱和和单不饱和脂肪酸组成。通过研究确定了 Cyanobacterium sp. IPPAS B-1200 的最佳生长条件。这种微生物具有很好的生产生物柴油的潜力，因为它是饱和和单不饱和中链长脂肪酸的生产者。
