Co-Cultivation of Leptolyngbya tenuis (Cyanobacteria) and Chlorella ellipsoidea (Green alga) for Biodiesel Production, Carbon Sequestration, and Cadmium Accumulation.

The co-cultivation approach using cyanobacteria-Leptolyngbya tenuis and green alga-Chlorella ellipsoidea demonstrated in the present study showed additive and synergistic effects on biomass yield, biomass productivity, lipid yield, lipid productivity, CO fixation, and cadmium bioremediation efficiency. The results of co-culture in batch mode revealed about 2-3 times increase in biomass and two times increase in total lipid, when compared to the pure culture batches. The results revealed that co-cultures exhibited significantly high CO fixation rate of 2.63  ±  0.09 g/L/d, which is 1.5-2 times better than monocultures (P < 0.05). To explore the bioaccumulation of cadmium by co-cultures and pure cultures, different concentrations of cadmium nitrate was used in flask trials. Cadmium accumulation was observed in the order: co-culture (74%, 0.37 mg/L) > Chlorella (58%, 0.29 mg/L) > Leptolyngbya (50%, 0.25 mg/L) (P < 0.05). In addition, fatty acid composition, CHNS analysis, biodiesel characterization, and biochemical compositions were also determined using co-culture method. The maximum biomass yield, productivity, lipid content, and CO fixation rate in cadmium induced co-culture were 3.95  ±  0.13 g/L, 258.88  ±  15.75 mg/L/d, 41.43  ±  0.71%, and 3.21  ±  0.20 g/L/d, respectively which is 1.2, 1.3, 2.3, and 1.2 times higher than the control (P < 0.05). Cadmium induced changes in growth and lipid yield using co-culture suggests cost-effective and eco-friendly production of biodiesel and carbon mitigation.