Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a La Presa de San José, 2055, Lomas 4 sección, A.C, 78216, San Luis Potosí, Mexico.
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 2001, 62209, Cuernavaca, Morelos, Mexico.
Plant Physiol Biochem. 2020 Nov;156:494-503. doi: 10.1016/j.plaphy.2020.09.035. Epub 2020 Oct 6.
During plant-microbe interactions, polyamines participate in the plant defense response. Previously, we reported that silencing of ADC genes in Arabidopsis thaliana causes a drastic reduction of polyamine levels as well as increments in reactive oxygen species content. In this study, we examined the response of the adc-silenced line to Botrytis cinerea and Pseudomonas syringae infection. The adc-silenced line was more susceptible to Botrytis cinerea, showing larger lesion length and a higher incidence of fungal infection. Pre-treatments with putrescine reestablished the response of the adc-silenced line to Botrytis cinerea, resulting in a similar phenotype to the parental plant. Expression levels of defense-related genes were analyzed during fungal infection showing that the salicylic acid-induced gene PR1 was up-regulated, while the jasmonic acid-related genes LOX3 and PDF1.2, as well as, the camalexin biosynthetic gene PAD3 were down-regulated in the adc-silenced line. Furthermore, methyl jasmonate pre-treatments reduced Botrytis cinerea infection in the adc-silenced line. On the other hand, the adc-silenced line showed an increased resistance to Pseudomonas syringae infection. SA-related genes such as PR1, ZAT1.2, WRKY54 and WRKY70 were highly expressed in the adc-silenced line upon bacterial interaction. Our data show that the adc-silenced line has altered the defense-response against Botrytis cinerea and Pseudomonas syringae, that is consistent with deregulation of SA- and JA-mediated response pathways.
在植物-微生物相互作用中，多胺参与植物防御反应。以前，我们报道过拟南芥 ADC 基因沉默会导致多胺水平大幅降低和活性氧含量增加。在这项研究中，我们检查了沉默 ADC 基因的拟南芥对线虫和丁香假单胞菌感染的反应。沉默 ADC 基因的系更容易受到线虫的感染，表现出更大的病变长度和更高的真菌感染发生率。腐胺预处理恢复了沉默 ADC 基因的对线虫的反应，导致与亲本植物相似的表型。在真菌感染过程中分析了防御相关基因的表达水平，结果表明水杨酸诱导的基因 PR1 上调，而茉莉酸相关基因 LOX3 和 PDF1.2 以及 camalexin 生物合成基因 PAD3 在沉默 ADC 基因的系中下调。此外，茉莉酸甲酯预处理降低了沉默 ADC 基因的系对线虫的感染。另一方面，沉默 ADC 基因的系对丁香假单胞菌感染表现出增强的抗性。SA 相关基因，如 PR1、ZAT1.2、WRKY54 和 WRKY70，在细菌相互作用时在沉默 ADC 基因的系中高度表达。我们的数据表明，沉默 ADC 基因的系对线虫和丁香假单胞菌的防御反应发生了改变，这与 SA 和 JA 介导的反应途径的失调一致。
