Avgeri Ioanna, Zeliou Konstantina, Petropoulos Spyridon A, Bebeli Penelope J, Papasotiropoulos Vasileios, Lamari Fotini N
Laboratory of Pharmacognosy and Natural Products, Department of Pharmacy, University of Patras, 26 504 Patras, Greece.
Laboratory of Vegetable Production, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou, 38 446 Volos, Greece.
Antioxidants (Basel). 2020 Oct 9;9(10):967. doi: 10.3390/antiox9100967.
In order to assess the diversity of Greek garlic ( L.) landraces, 34 genotypes including commercial ones were grown in the same field and their content in organosulfur compounds, pyruvate, total sugars, and total phenolics, alongside antioxidant capacity, was determined. The organosulfur compounds were studied by Gas Chromatography-Mass Spectrometry (GC-MS) after ultrasound-assisted extraction in ethyl acetate, identifying 2-vinyl-4H-1,3-dithiin and 3-vinyl-4H-1,2-dithiin as the predominant compounds, albeit in different ratios among genotypes. The bioactivity and the polar metabolites were determined in hydromethanolic extracts. A great variability was revealed, and nearly one-third of landraces had higher concentration of compounds determining bioactivity and organoleptic traits than the imported ones. We recorded strong correlations between pyruvate and total organosulfur compounds, and between antioxidant capacity and phenolics. In conclusion, chemical characterization revealed great genotype-dependent variation in the antioxidant properties and the chemical characters, identifying specific landraces with superior traits and nutritional and pharmaceutical value.
为了评估希腊大蒜（L.）地方品种的多样性，34种基因型（包括商业品种）在同一块田地中种植，并测定了它们在有机硫化合物、丙酮酸、总糖和总酚方面的含量，以及抗氧化能力。在超声辅助下用乙酸乙酯提取后，通过气相色谱-质谱联用仪（GC-MS）对有机硫化合物进行研究，确定2-乙烯基-4H-1,3-二噻英和3-乙烯基-4H-1,2-二噻英为主要化合物，尽管不同基因型之间比例不同。在氢甲醇提取物中测定生物活性和极性代谢物。结果显示出很大的变异性，近三分之一的地方品种中决定生物活性和感官特性的化合物浓度高于进口品种。我们记录到丙酮酸与总有机硫化合物之间、抗氧化能力与酚类之间存在强相关性。总之，化学表征揭示了抗氧化特性和化学特征在很大程度上依赖于基因型的变异，鉴定出了具有优良性状以及营养和药用价值的特定地方品种。
