Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan.
Molecules. 2020 Oct 10;25(20):4608. doi: 10.3390/molecules25204608.
is widely used in herbal medicine. However, the anti-cancer activity of extract (CAt extract) has not been clarified in hepatocellular carcinoma. In the study, we elucidated the anti-hepatoma capacity of CAt extract on HCC in vitro and in vivo. To explore the anti-hepatoma mechanisms of the CAt extract in vitro, HCC and normal cells were treated with the CAt extract, which showed marked inhibitory effects on HCC cells in a dose-dependent manner; in contrast, the CAt extract treatment was less cytotoxic to normal cells. In addition, our results indicate that the CAt extract induced apoptosis via caspase-dependent and independent apoptosis pathways. Furthermore, the CAt extract inhibited HCC tumor cell growth by restraining cell cycle progression, and it reduced the signaling of the AKT, ERK1/2, and p38 pathways. In the xenograft model, the CAt extract suppressed HCC tumor cell growth and prolonged lifespan by inhibiting PCNA protein expression, repressing part of the VEGF-induced autocrine pathway, and triggering strong expression of cleaved caspase-3, which contributed to cell apoptosis. Moreover, the CAt extract did not induce any obvious changes in pathological morphology or body weight, suggesting it had no toxicity. CAt extract exerted anti-tumor effects on HCC in vitro and in vivo. Thus, CAt extract could be used as a potential anti-cancer therapeutic agent against HCC.
在草药医学中被广泛应用。然而， 提取物（CAt 提取物）在肝细胞癌中的抗癌活性尚未得到阐明。在这项研究中，我们在体外和体内阐明了 CAt 提取物对肝癌的抗癌能力。为了探索 CAt 提取物在体外的抗癌机制，我们用 CAt 提取物处理 HCC 和正常细胞，结果表明 CAt 提取物呈剂量依赖性地显著抑制 HCC 细胞；相比之下，CAt 提取物对正常细胞的毒性较小。此外，我们的结果表明，CAt 提取物通过 caspase 依赖和非依赖的凋亡途径诱导细胞凋亡。此外，CAt 提取物通过抑制细胞周期进程来抑制 HCC 肿瘤细胞的生长，并降低 AKT、ERK1/2 和 p38 通路的信号。在异种移植模型中，CAt 提取物通过抑制 PCNA 蛋白表达、抑制部分 VEGF 诱导的自分泌途径以及触发强烈的 cleaved caspase-3 表达来抑制 HCC 肿瘤细胞的生长并延长寿命，从而促进细胞凋亡。此外，CAt 提取物没有引起明显的病理形态或体重变化，表明其没有毒性。CAt 提取物在体外和体内对 HCC 均具有抗肿瘤作用。因此，CAt 提取物可用作针对 HCC 的潜在抗癌治疗剂。
