Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology in Warsaw, ul. Ks. Trojdena 4, PL-02-109 Warsaw, Poland.
Bioinformatics Laboratory, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, PL-61-614 Poznan, Poland.
Nucleic Acids Res. 2021 Jan 8;49(D1):D76-D81. doi: 10.1093/nar/gkaa867.
Deoxyribozymes, DNA enzymes or simply DNAzymes are single-stranded oligo-deoxyribonucleotide molecules that, like proteins and ribozymes, possess the ability to perform catalysis. Although DNAzymes have not yet been found in living organisms, they have been isolated in the laboratory through in vitro selection. The selected DNAzyme sequences have the ability to catalyze a broad range of chemical reactions, utilizing DNA, RNA, peptides or small organic compounds as substrates. DNAmoreDB is a comprehensive database resource for DNAzymes that collects and organizes the following types of information: sequences, conditions of the selection procedure, catalyzed reactions, kinetic parameters, substrates, cofactors, structural information whenever available, and literature references. Currently, DNAmoreDB contains information about DNAzymes that catalyze 20 different reactions. We included a submission form for new data, a REST-based API system that allows users to retrieve the database contents in a machine-readable format, and keyword and BLASTN search features. The database is publicly available at https://www.genesilico.pl/DNAmoreDB/.
脱氧核酶、DNA 酶或简称 DNA 酶是单链寡脱氧核糖核苷酸分子，与蛋白质和核酶一样，具有催化能力。虽然脱氧核酶尚未在生物体内发现，但已通过体外选择在实验室中分离出来。所选 DNA 酶序列具有催化广泛的化学反应的能力，利用 DNA、RNA、肽或小分子有机化合物作为底物。DNAmoreDB 是一个综合性的 DNA 酶数据库资源，收集和组织以下类型的信息：序列、选择程序的条件、催化反应、动力学参数、底物、辅助因子、结构信息（如有）和文献参考。目前，DNAmoreDB 包含了催化 20 种不同反应的 DNA 酶的信息。我们还包含了一个新数据的提交表单、一个基于 REST 的 API 系统，允许用户以机器可读的格式检索数据库内容，以及关键字和 BLASTN 搜索功能。该数据库可在 https://www.genesilico.pl/DNAmoreDB/ 上公开获取。
