Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6126-6132. doi: 10.1099/ijsem.0.004507. Epub 2020 Oct 15.
A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated strain IMCC25678, was isolated from an artificial freshwater reservoir, Chungju Lake, in the Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC25678 belongs to the genus with ≤98.7 % sequence similarities to species. Whole genome sequencing of strain IMCC25678 revealed a 3.9 Mbp genome size with a DNA G+C content of 42.2 mol%. The IMCC25678 genome shared ≤89.7 % average nucleotide identity and ≤21.4 % digital DNA-DNA hybridization values with closely related species of the genus , indicating that the strain represents a novel species. Summed feature 3 (C 6 and/or C 7), iso-C and iso-C 3-OH were found to be the predominant cellular fatty acid constituents in the strain. The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unidentified phosphoglycolipid, one unidentified sphingolipid and three unidentified polar lipids. Based on the phylogenetic and phenotypic characteristics, strain IMCC25678 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is IMCC25678 (=KACC 19485=NBRC 113130).
一株革兰氏阴性、杆状、需氧、不具鞭毛、化能异养的细菌，被命名为 IMCC25678 菌株，从韩国忠州市的人工淡水水库钟珠湖中分离得到。16S rRNA 基因序列分析表明，菌株 IMCC25678 属于 属，与 种的序列相似性≤98.7%。对菌株 IMCC25678 的全基因组测序表明，其基因组大小为 3.9 Mbp，GC 含量为 42.2mol%。IMCC25678 基因组与该属中密切相关的种的平均核苷酸同一性≤89.7%，数字 DNA-DNA 杂交值≤21.4%，表明该菌株代表了一个新的种。菌株中主要的细胞脂肪酸成分是 summed feature 3 (C 6 和/或 C 7)、iso-C 和 iso-C 3-OH。主要的呼吸醌是 MK-7。主要的极性脂是磷脂酰乙醇胺、一种未鉴定的磷酸甘油酯、一种未鉴定的鞘脂和三种未鉴定的极性脂。根据系统发育和表型特征，菌株 IMCC25678 被认为是 属中的一个新种，建议命名为 sp. nov.。其模式菌株是 IMCC25678（=KACC 19485=NBRC 113130）。
