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平行的植物 TOR 动力学全局分析揭示了 LARP1 在翻译中的保守作用。

Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in translation.

机构信息

Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, United States.

Plant Gene Expression Center, U.S. Department of Agriculture Agricultural Research Service, Albany, United States.

出版信息

Elife. 2020 Oct 15;9:e58795. doi: 10.7554/eLife.58795.

DOI:10.7554/eLife.58795
PMID:33054972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7584452/
Abstract

Target of rapamycin (TOR) is a protein kinase that coordinates eukaryotic metabolism. In mammals, TOR specifically promotes translation of ribosomal protein (RP) mRNAs when amino acids are available to support protein synthesis. The mechanisms controlling translation downstream from TOR remain contested, however, and are largely unexplored in plants. To define these mechanisms in plants, we globally profiled the plant TOR-regulated transcriptome, translatome, proteome, and phosphoproteome. We found that TOR regulates ribosome biogenesis in plants at multiple levels, but through mechanisms that do not directly depend on 5' oligopyrimidine tract motifs (5'TOPs) found in mammalian RP mRNAs. We then show that the TOR-LARP1-5'TOP signaling axis is conserved in plants and regulates expression of a core set of eukaryotic 5'TOP mRNAs, as well as new, plant-specific 5'TOP mRNAs. Our study illuminates ancestral roles of the TOR-LARP1-5'TOP metabolic regulatory network and provides evolutionary context for ongoing debates about the molecular function of LARP1.

摘要

雷帕霉素靶蛋白（TOR）是一种蛋白激酶，可协调真核生物的新陈代谢。在哺乳动物中，当氨基酸可用于支持蛋白质合成时，TOR 特异性地促进核糖体蛋白（RP）mRNA 的翻译。然而，控制 TOR 下游翻译的机制仍存在争议，在植物中也基本没有得到探索。为了在植物中定义这些机制，我们对植物 TOR 调节的转录组、翻译组、蛋白质组和磷酸化蛋白质组进行了全面分析。我们发现，TOR 在多个水平上调节植物核糖体的生物发生，但不直接依赖于在哺乳动物 RP mRNA 中发现的 5'寡嘧啶 tract 基序（5'TOPs）。然后我们表明，TOR-LARP1-5'TOP 信号轴在植物中是保守的，调节一组核心的真核 5'TOP mRNA 以及新的植物特异性 5'TOP mRNA 的表达。我们的研究阐明了 TOR-LARP1-5'TOP 代谢调控网络的古老作用，并为关于 LARP1 分子功能的持续争论提供了进化背景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/efa28b4b3ea9/elife-58795-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/08f8f5f39415/elife-58795-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/2697be541551/elife-58795-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/4f0903cc6ca1/elife-58795-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/8b541e461f69/elife-58795-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/bdc96ab84296/elife-58795-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/efa28b4b3ea9/elife-58795-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/08f8f5f39415/elife-58795-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/2697be541551/elife-58795-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/4f0903cc6ca1/elife-58795-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/8b541e461f69/elife-58795-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/bdc96ab84296/elife-58795-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/186f/7584452/efa28b4b3ea9/elife-58795-fig6.jpg

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