Bauer J
J Chromatogr. 1987 Jul 17;418:359-83. doi: 10.1016/0378-4347(87)80015-4.
There are several kinds of cell electrophoresis. The most important are free-flow electrophoresis, scaled-up free-flow electrophoresis and column electrophoresis. All kinds of cell electrophoresis, especially free-flow electrophoresis, have been improved to a very high standard of separation accuracy, and their application possibilities are extended when antigen-specific electrophoretic cell separation is performed, or when cell electrophoresis is combined with other physical methods for cell separation. Cell electrophoresis and other physical cell separation methods have the advantage that the functional state of the cells remains virtually unchanged during the isolation procedure. With the help of cell electrophoresis monocytes, T-lymphocytes, platelets and other cells from human peripheral blood could be purified. Other human cells were enriched for immunological characterization. Furthermore, cells that secreted human plasminogen activators or human antibodies were electrophoresed to give cell fractions with increased frequencies of the cells of interest.
细胞电泳有几种类型。最重要的是自由流动电泳、放大的自由流动电泳和柱电泳。各种细胞电泳，尤其是自由流动电泳，已被改进到非常高的分离精度标准，并且当进行抗原特异性电泳细胞分离或细胞电泳与其他物理细胞分离方法结合时，其应用可能性会得到扩展。细胞电泳和其他物理细胞分离方法的优点是在分离过程中细胞的功能状态几乎保持不变。借助细胞电泳，可以纯化人外周血中的单核细胞、T淋巴细胞、血小板和其他细胞。其他人类细胞被富集用于免疫学表征。此外，对分泌人纤溶酶原激活剂或人抗体的细胞进行电泳，以得到感兴趣细胞频率增加的细胞组分。
