Hoffman D R
J Allergy Clin Immunol. 1987 Sep;80(3 Pt 1):307-13. doi: 10.1016/0091-6749(87)90036-4.
Pure venoms from yellow jackets and bees were used to evaluate denaturing and nondenaturing immunoblot techniques and to compare results of IgE antibody-stained immunoblots to RAST with highly purified allergens. Significant differences in IgE antibody binding were observed among all three techniques, suggesting that tertiary conformation is a major factor in the allergenic determinants of venom allergens. IgG antibodies from hyperimmunized animals and from specimens obtained from allergic patients before and after venom immunotherapy appeared to exhibit less conformational dependence for binding. The conformational dependence of IgE antibody binding to bee and yellow jacket phospholipases was confirmed by direct and inhibition RAST studies. The allergens studied were all single polypeptide chains.
用黄蜂和蜜蜂的纯毒液来评估变性和非变性免疫印迹技术，并将IgE抗体染色免疫印迹的结果与使用高度纯化变应原的放射性变应原吸附试验（RAST）的结果进行比较。在所有这三种技术中均观察到IgE抗体结合存在显著差异，这表明三级构象是毒液变应原的变应原决定簇中的一个主要因素。来自经超免疫动物的IgG抗体以及来自变应原免疫疗法前后从过敏患者获得的标本中的IgG抗体，其结合似乎表现出对构象的依赖性较小。通过直接和抑制性RAST研究证实了IgE抗体与蜜蜂和黄蜂磷脂酶结合的构象依赖性。所研究的变应原均为单条多肽链。
