School of Science, Western Sydney University, Penrith, New South Wales, Australia.
Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Westmead, New South Wales, Australia.
J Biophotonics. 2021 Jan;14(1):e202000340. doi: 10.1002/jbio.202000340. Epub 2020 Oct 26.
Photodynamic therapy (PDT) with Rose Bengal has previously achieved eradication of Trichophyton rubrum infections causing toenail onychomycosis; however, its antifungal activity against other clinically relevant dermatophytes has yet to be studied. Here, we test the efficacy of PDT using Rose Bengal (140 μM) and 532 nm irradiation (101 J/cm ) against Trichophyton mentagrophytes and Trichophyton interdigitale spores, in comparison to T. rubrum. A significant reduction (>99%) of T. mentagrophytes and T. interdigitale was observed, while actual eradication of viable T. rubrum was achieved (99.99%). Laser irradiation alone inhibited growth of T. rubrum (55.2%) and T. mentagrophytes (45.2%) significantly more than T. interdigitale (25.5%) (P = .0086), which may indicate an increased presence of fungal pigments, xanthomegnin and melanin. The findings suggest that Rose Bengal-PDT can act against a broader spectrum of fungal pathogens, and with continued development may be employed in a wider range of clinical antifungal applications.
光动力疗法（PDT）用孟加拉玫瑰红已被证实可根除导致趾甲甲真菌病的红色毛癣菌感染；然而，其对其他临床上相关的皮肤癣菌的抗真菌活性尚未得到研究。在这里，我们测试了用孟加拉玫瑰红（140μM）和 532nm 照射（101J/cm）治疗毛癣菌和须癣毛癣菌孢子的 PDT 的功效，与红色毛癣菌进行了比较。观察到毛癣菌和须癣毛癣菌的显著减少（>99%），而实际根除了有活力的红色毛癣菌（99.99%）。单独的激光照射显著抑制了红色毛癣菌（55.2%）和毛癣菌（45.2%）的生长，而对须癣毛癣菌（25.5%）的抑制作用较小（P =.0086），这可能表明真菌色素黄烷酮和黑色素的存在增加。这些发现表明，孟加拉玫瑰红-PDT 可以作用于更广泛的真菌病原体，并且随着进一步的发展，可能会在更广泛的临床抗真菌应用中得到应用。
