Martel-Pelletier J, Pelletier J P
J Rheumatol. 1987 May;14 Spec No:38-40.
In this study, we measured the levels of neutral metallo- and serine proteases in human osteoarthritic synovium. These enzymes degrade both collagen and proteoglycan macromolecules. They were analyzed by tissue culture methodology and direct extraction. We have demonstrated that in human osteoarthritic synovium, there is a correlation between neutral enzyme activity and the severity of synovial inflammation. Tissue culture studies have shown that the human osteoarthritic synovial membrane produces metalloproteases, such as collagenase, proteoglycanase and gelatinase. These enzymes were further characterized by their molecular weight. Extracts of osteoarthritic synovial tissues showed the presence of serine proteases, with apparent Mr of 25,000.
在本研究中，我们测量了人类骨关节炎滑膜中中性金属蛋白酶和丝氨酸蛋白酶的水平。这些酶可降解胶原蛋白和蛋白聚糖大分子。通过组织培养方法和直接提取对它们进行了分析。我们已经证明，在人类骨关节炎滑膜中，中性酶活性与滑膜炎症的严重程度之间存在相关性。组织培养研究表明，人类骨关节炎滑膜组织会产生金属蛋白酶，如胶原酶、蛋白聚糖酶和明胶酶。这些酶通过其分子量进一步表征。骨关节炎滑膜组织提取物显示存在丝氨酸蛋白酶，其表观分子量为25,000。
