Allison D P, Daw C S, Rorvik M C
J Microsc. 1987 Jul;147(Pt 1):103-8. doi: 10.1111/j.1365-2818.1987.tb02822.x.
A simple and inexpensive slam freezing device has been constructed that allows consistently good rapid freezing of uncryoprotected tissue. The device employs a dry liquid-nitrogen-cooled cooper surface and a slamming plunger designed to provide rapid and uniform tissue to metal contact.
已构建了一种简单且廉价的急冻装置,该装置能始终如一地实现对未添加冷冻保护剂的组织进行良好的快速冷冻。该装置采用液氮冷却的干式铜质表面以及一个撞击式柱塞,其设计目的是使组织与金属能够快速且均匀地接触。