A simple and inexpensive liquid helium cooled 'slam freezing' device.
作者信息
Heath I B
出版信息
J Microsc. 1984 Jul;135(Pt 1):75-82. doi: 10.1111/j.1365-2818.1984.tb04650.x.
Abstract
A liquid helium cooled copper block device has been designed for the rapid freezing of biological material. The apparatus differs from previous designs in being easily constructed from readily available components. It incorporates a novel and simplified specimen carrying system. Construction of this apparatus is possible for any laboratory and makes the advantages of freeze substitution reliably available for many cell types at minimal cost.
摘要
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